Jacobson E B, L'age-Stehr J, Herzenberg L A
J Exp Med. 1970 Jun 1;131(6):1109-20. doi: 10.1084/jem.131.6.1109.
Congenic mice, differing genetically only at the loci coding for immunoglobulin H chain (or Fc) structures, have been used to study cell interactions in the 7S (gammaG(2a)) antibody response to sheep erythrocytes (SRBC), as detected by the Jerne plaque-forming cell (PFC) method. The interaction between thymus and bone marrow cells was studied in adult thymectomized irradiated recipients, protected with syngeneic bone marrow and injected with thymus cells from the partner congenic strain. All of the gammaG(2a) PFC detected in the spleens of these mice were of bone marrow allotype. Adoptive secondary immune responses were then studied to determine whether a similar interaction between memory cells and bone marrow derived cells could be detected. Primed spleen cells from the partner congenic strain, or a subpopulation of these cells obtained by BSA density gradient fractionation, were injected into irradiated recipients alone, or together with syngeneic nonimmune spleen or bone marrow cells. All gammaG(2a) PFC detected in these experiments were of primed cell allotype. There was no evidence that antibody forming cell precursors in normal spleen or bone marrow participate in the adoptive secondary immune response detected 7 days after transfer of primed spleen cells. This was true regardless of whether the bone marrow cells were injected at the time of transfer, or were injected 1-2 wk earlier and allowed to become established in the spleens of recipient mice. Although no specific cell interaction was seen, bone marrow (and, to a lesser degree, normal spleen) cells were found to have a nonspecific enhancing effect on the adoptive secondary response when they were injected together with the primed spleen cells. This enhancement was not evident if the bone marrow cells were injected 1 or 2 wk prior to primed cell transfer.
同基因小鼠，其基因差异仅在于编码免疫球蛋白H链（或Fc）结构的位点，已被用于研究通过耶尔恩空斑形成细胞（PFC）方法检测到的对绵羊红细胞（SRBC）的7S（γG(2a)）抗体反应中的细胞相互作用。在成年胸腺切除并经照射的受体中研究胸腺细胞与骨髓细胞之间的相互作用，这些受体用同基因骨髓进行保护，并注射来自配对同基因品系的胸腺细胞。在这些小鼠脾脏中检测到的所有γG(2a) PFC均为骨髓同种异型。然后研究了过继性二次免疫反应，以确定是否能检测到记忆细胞与骨髓来源细胞之间的类似相互作用。将来自配对同基因品系的致敏脾细胞，或通过牛血清白蛋白密度梯度分级分离获得的这些细胞的亚群，单独注射到经照射的受体中，或与同基因非免疫脾细胞或骨髓细胞一起注射。在这些实验中检测到的所有γG(2a) PFC均为致敏细胞同种异型。没有证据表明正常脾脏或骨髓中的抗体形成细胞前体参与了致敏脾细胞转移7天后检测到的过继性二次免疫反应。无论骨髓细胞是在转移时注射，还是在1 - 2周前注射并使其在受体小鼠脾脏中定植，情况都是如此。尽管未观察到特异性细胞相互作用，但当骨髓细胞（以及程度较轻的正常脾脏细胞）与致敏脾细胞一起注射时，发现它们对过继性二次反应有非特异性增强作用。如果在致敏细胞转移前1或2周注射骨髓细胞，这种增强作用不明显。
