交联转运核糖核酸在翻译过程的所有步骤中都发挥作用。

Cross-linked transfer RNA functions in all steps of the translation process.

作者信息

Chaffin L J, Omilianowski D R, Bock R M

出版信息

Science. 1971 May 21;172(3985):854-5. doi: 10.1126/science.172.3985.854.

DOI:10.1126/science.172.3985.854

Abstract

A specific photochemical reaction between 4-thiouridine and cytosine cross-links two arms of transfer RNA. This cross-link, introduced into phenylalanine transfer RNA and arginine transfer RNA, limits the conformational freedom of the molecule. Both modified transfer RNA's are capable of functioning in all steps of protein synthesis with this restraint on allowable conformations.

摘要

4-硫尿苷与胞嘧啶之间的特定光化学反应使转运RNA的两条臂发生交联。这种交联引入到苯丙氨酸转运RNA和精氨酸转运RNA中，限制了分子的构象自由度。在允许构象受到这种限制的情况下，两种修饰后的转运RNA都能够在蛋白质合成的所有步骤中发挥作用。