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Nuclease activity associated with the Ustilago maydis virus induced killer proteins.

作者信息

Levine R, Koltin Y, Kandel J

出版信息

Nucleic Acids Res. 1979 Aug 24;6(12):3717-31. doi: 10.1093/nar/6.12.3717.

DOI:10.1093/nar/6.12.3717
PMID:493119
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327973/
Abstract

An in vitro nuclease activity was found to be associated with the purified killer proteins of Ustilago maydis. The proteins are effective against single stranded RNA, single and double stranded DNA. Endonucleolytic activity was confirmed by cleavage of circular molecules of 0x174 and PM2. Double stranded RNA did not appear to serve as a substrate.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77d1/327973/b0602c9a2458/nar00453-0020-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77d1/327973/36c4f5f71195/nar00453-0012-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77d1/327973/530e8c77dade/nar00453-0018-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77d1/327973/7f1367061b32/nar00453-0019-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77d1/327973/b0602c9a2458/nar00453-0020-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77d1/327973/36c4f5f71195/nar00453-0012-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77d1/327973/530e8c77dade/nar00453-0018-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77d1/327973/7f1367061b32/nar00453-0019-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/77d1/327973/b0602c9a2458/nar00453-0020-a.jpg

相似文献

1
Nuclease activity associated with the Ustilago maydis virus induced killer proteins.
Nucleic Acids Res. 1979 Aug 24;6(12):3717-31. doi: 10.1093/nar/6.12.3717.
2
Synthesis and processing of killer toxin from Ustilago maydis virus P4.来自玉米黑粉菌病毒P4的杀伤毒素的合成与加工
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RNA polymerase activity in virions from Ustilago maydis.来自玉米黑粉菌的病毒粒子中的RNA聚合酶活性。
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The Ustilago maydis virally encoded KP1 killer toxin.玉米黑粉菌病毒编码的KP1杀伤毒素。
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J Biol Chem. 1981 May 25;256(10):5087-94.
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Nucleic acid-binding glycoproteins which solubilize nucleic acids in dilute acid. Re-examination of the Ustilago maydis glycoproteins.
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Inheritance of killer phenotypes and double-stranded RNA in Ustilago maydis.玉米黑粉菌中杀手表型和双链RNA的遗传
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8
Characterization of Ustilago Ribonuclease U2. Effects of chemical modification at glutamic acid-61 and cystine-1 and of organic solvents on the enzymatic activity.
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Ustilago maydis KP6 killer toxin: structure, expression in Saccharomyces cerevisiae, and relationship to other cellular toxins.玉蜀黍黑粉菌KP6杀伤毒素：结构、在酿酒酵母中的表达及其与其他细胞毒素的关系。
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10
A ribonuclease H from Ustilago maydis. Properties, mode of action and substrate specificity of the enzyme.来自玉米黑粉菌的核糖核酸酶H。该酶的性质、作用方式及底物特异性
Eur J Biochem. 1974 Sep 16;47(3):499-507. doi: 10.1111/j.1432-1033.1974.tb03718.x.

引用本文的文献

1
The exclusion among the Ustilago viruses - A dsRNA restriction modification system?是否存在 Ustilago 病毒的排除现象——dsRNA 限制修饰系统？
Curr Genet. 1980 Dec;2(3):185-92. doi: 10.1007/BF00435684.
2
Dual toxin specificities and the exclusion relations among the Ustilago dsRNA viruses.双毒素特异性和 Ustilago dsRNA 病毒之间的排除关系。
Curr Genet. 1982 Jul;5(2):127-36. doi: 10.1007/BF00365703.
3
Assignment of functions to segments of the DsRNA genome of the Ustilago virus.黑粉菌病毒双链RNA基因组各片段的功能分配

本文引用的文献

1
Killer Phenomenon in USTILAGO MAYDIS: The Organization of the Viral Genome.玉米黑粉菌中的杀伤现象：病毒基因组的组织。
Genetics. 1978 Feb;88(2):267-76. doi: 10.1093/genetics/88.2.267.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
Mol Gen Genet. 1980 Apr;178(1):173-8. doi: 10.1007/BF00267226.
4
Virus-encoded toxin of Ustilago maydis: two polypeptides are essential for activity.玉米黑粉菌的病毒编码毒素：两种多肽对活性至关重要。
Mol Cell Biol. 1987 Jan;7(1):470-7. doi: 10.1128/mcb.7.1.470-477.1987.
5
Ustilago maydis KP6 killer toxin: structure, expression in Saccharomyces cerevisiae, and relationship to other cellular toxins.玉蜀黍黑粉菌KP6杀伤毒素：结构、在酿酒酵母中的表达及其与其他细胞毒素的关系。
Mol Cell Biol. 1990 Apr;10(4):1373-81. doi: 10.1128/mcb.10.4.1373-1381.1990.
J Biol Chem. 1969 Aug 25;244(16):4406-12.
4
Compatibility reactions on solid medium and interstrain inhibition in Ustilago maydis.玉米黑粉菌在固体培养基上的亲和反应及菌株间抑制作用
Genetics. 1968 Nov;60(3):461-74. doi: 10.1093/genetics/60.3.461.
5
Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳分离十种呼肠孤病毒基因组片段。
J Virol. 1968 Oct;2(10):986-91. doi: 10.1128/JVI.2.10.986-991.1968.
6
Analytical fractionation of plant and animal proteins by gel electrofocusing.通过凝胶电聚焦对植物和动物蛋白质进行分析分级分离。
J Chromatogr. 1968 Aug 27;36(3):362-5. doi: 10.1016/s0021-9673(01)92959-0.
7
Replication of bacteriophage PM2 deoxyribonucleic acid: a closed circular double-stranded molecule.噬菌体PM2脱氧核糖核酸的复制：一种闭环双链分子。
J Mol Biol. 1971 Mar 28;56(3):597-621. doi: 10.1016/0022-2836(71)90404-9.
8
Structure of the DNA of bacteriophage phiX174. VII. Methylation.噬菌体φX174的DNA结构。VII. 甲基化
J Mol Biol. 1970 Oct 28;53(2):251-9. doi: 10.1016/0022-2836(70)90298-6.
9
Further analysis of the altered secondary structure of superhelical DNA. Sensitivity to methylmercuric hydroxide a chemical probe for unpaired bases.超螺旋DNA二级结构改变的进一步分析。对氢氧化甲基汞的敏感性——一种针对未配对碱基的化学探针。
J Mol Biol. 1973 Sep 25;79(3):451-70. doi: 10.1016/0022-2836(73)90398-7.
10
Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.利用分析型琼脂糖-溴化乙锭电泳检测副流感嗜血杆菌中的两种限制性内切酶活性。
Biochemistry. 1973 Jul 31;12(16):3055-63. doi: 10.1021/bi00740a018.