Podila G K, Bozarth R F, Flurkey W H
Dept. of Life Sciences, Indiana State University, Terre Haute 47809.
Biochem Biophys Res Commun. 1987 Dec 16;149(2):391-7. doi: 10.1016/0006-291x(87)90379-2.
The synthesis of toxin protein from Ustilago maydis virus (UmV) strain P4 was studied in vitro and in vivo. The protein synthesized in vitro and in vivo has a molecular weight of approximately 30 kd whereas the native toxin has a molecular weight of about 12 kd. In the presence of protease inhibitors and glycosylation inhibitors, toxin protein synthesized in vivo showed higher molecular weight products that could be immunoprecipitated with toxin antibodies. These results suggest that the UmV P4 toxin protein is synthesized as a preprotein, which upon processing results in the 12 kd secreted form toxin.
对玉米黑粉菌病毒（UmV）P4株毒素蛋白的合成进行了体外和体内研究。体外和体内合成的蛋白分子量约为30kd，而天然毒素分子量约为12kd。在蛋白酶抑制剂和糖基化抑制剂存在的情况下，体内合成的毒素蛋白显示出分子量更高的产物，这些产物可用毒素抗体进行免疫沉淀。这些结果表明，UmV P4毒素蛋白以前体蛋白形式合成，加工后产生12kd的分泌型毒素。
