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玉米黑粉菌的病毒编码毒素：两种多肽对活性至关重要。

Virus-encoded toxin of Ustilago maydis: two polypeptides are essential for activity.

作者信息

Peery T, Shabat-Brand T, Steinlauf R, Koltin Y, Bruenn J

出版信息

Mol Cell Biol. 1987 Jan;7(1):470-7. doi: 10.1128/mcb.7.1.470-477.1987.

DOI:10.1128/mcb.7.1.470-477.1987
PMID:3561397
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC365090/
Abstract

Cells of Ustilago maydis containing double-stranded RNA viruses secrete a virus-encoded toxin to which other cells of the same species and related species are sensitive. Mutants affected in the expression of the KP6 toxin were characterized, and all were viral mutants. A temperature-sensitive nonkiller mutant indicated that the toxin consists of two polypeptides, 12.5K and 10K, that are essential for the toxic activity. The temperature-sensitive nonkiller mutant was affected in the expression of the 10K polypeptide, and its toxic activity was restored by the addition of the 10K polypeptide to its secreted inactive toxin. These results led to the reexamination of other mutants that were known to complement in vitro. Each was found to secrete one of the two polypeptides. Here we show for the first time that P6 toxin consists of two polypeptides that do not interact in solution, but both are essential for the toxic effect. Studies on the interaction between the two polypeptides indicated that there are no covalent or hydrogen bonds between the polypeptides. Toxin activity is not affected by the presence of 0.3 M NaCl in the toxin preparations and in the medium, suggesting that no electrostatic forces are involved in this interaction. Also, the two polypeptides do not share common antigenic determinants. The activity of the two polypeptides appears to be dependent on a sequential interaction with the target cell, and it is the 10K polypeptide that initiates the toxic effect. The similarity of the U. maydis virus-encoded toxin to that of Saccharomyces cerevisiae is discussed.

摘要

含有双链RNA病毒的玉米黑粉菌细胞会分泌一种病毒编码的毒素，同一物种和相关物种的其他细胞对该毒素敏感。对KP6毒素表达受影响的突变体进行了表征，结果发现它们都是病毒突变体。一个温度敏感的非杀伤性突变体表明，该毒素由两种多肽组成，分别为12.5K和10K，它们对毒性活性至关重要。该温度敏感的非杀伤性突变体在10K多肽的表达上受到影响，通过向其分泌的无活性毒素中添加10K多肽，其毒性活性得以恢复。这些结果促使对其他已知在体外具有互补作用的突变体进行重新研究。结果发现每个突变体都分泌两种多肽中的一种。在这里，我们首次表明P6毒素由两种在溶液中不相互作用的多肽组成，但两者对毒性作用都是必不可少的。对这两种多肽之间相互作用的研究表明，多肽之间不存在共价键或氢键。毒素制剂和培养基中存在0.3M NaCl时，毒素活性不受影响，这表明这种相互作用不涉及静电力。此外，这两种多肽不具有共同的抗原决定簇。这两种多肽的活性似乎依赖于与靶细胞的顺序相互作用，并且是10K多肽启动了毒性作用。文中还讨论了玉米黑粉菌病毒编码的毒素与酿酒酵母毒素的相似性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a806/365090/5aefb757dae3/molcellb00073-0494-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a806/365090/20a7341a6064/molcellb00073-0492-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a806/365090/47e747ecd92d/molcellb00073-0492-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a806/365090/5a9d3b5e8fef/molcellb00073-0493-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a806/365090/2b140e2ce520/molcellb00073-0493-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a806/365090/8d53005af136/molcellb00073-0494-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a806/365090/a1c34a073077/molcellb00073-0494-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a806/365090/5aefb757dae3/molcellb00073-0494-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a806/365090/20a7341a6064/molcellb00073-0492-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a806/365090/47e747ecd92d/molcellb00073-0492-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a806/365090/5a9d3b5e8fef/molcellb00073-0493-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a806/365090/2b140e2ce520/molcellb00073-0493-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a806/365090/8d53005af136/molcellb00073-0494-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a806/365090/a1c34a073077/molcellb00073-0494-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a806/365090/5aefb757dae3/molcellb00073-0494-c.jpg

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