质粒形成缺陷的细菌突变体：lon + 等位基因的需求
Bacterial mutants defective in plasmid formation: requirement for the lon + allele.
作者信息
Takano T
出版信息
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1469-73. doi: 10.1073/pnas.68.7.1469.
Abstract
Bacterial mutants defective in plasmid formation were selected for their inability to be transduced to chloramphenicol resistance by bacteriophage PlCM. The mutants isolated are indistinguishable from a lon mutant strain. Both the lon strain and the mutants isolated here show very poor lysogenization by P1 and very low transduction to Gal(+) by the plasmid formation of the lambdagal(8)-Nam7am53cI(857). The lon(+) gene function of the host bacteria is indispensable for plasmid formation, even though P1 and lambda(+) can grow normally on the lon strains and lambda(+) can be integrated normally in lon as well as in the lon(+) bacteria. Phage mutants that can persist as plasmids in lon strains were isolated from P1CM. The function of the lon(+) gene is discussed with regard to plasmid formation.
摘要
选择在质粒形成方面存在缺陷的细菌突变体，原因是它们无法被噬菌体P1CM转导获得氯霉素抗性。分离得到的突变体与lon突变菌株无法区分。lon菌株和此处分离得到的突变体在被P1溶源化方面表现很差，并且通过λgal(8)-Nam7am53cI(857)的质粒形成向Gal(+)的转导效率也非常低。宿主细菌的lon(+)基因功能对于质粒形成是不可或缺的，尽管P1和λ(+)能在lon菌株上正常生长，并且λ(+)能在lon以及lon(+)细菌中正常整合。从P1CM中分离出了能以质粒形式在lon菌株中持续存在的噬菌体突变体。本文讨论了lon(+)基因在质粒形成方面的功能。
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本文引用的文献
1
MINIATURE escherichia coli CELLS DEFICIENT IN DNA.DNA缺陷的微小大肠杆菌细胞
Proc Natl Acad Sci U S A. 1967 Feb;57(2):321-6. doi: 10.1073/pnas.57.2.321.
2
ANALYSIS OF A GENE CONTROLLING CELL DIVISION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI.大肠杆菌中一个控制细胞分裂及辐射敏感性的基因分析
J Bacteriol. 1964 Mar;87(3):720-6. doi: 10.1128/jb.87.3.720-726.1964.
3
A LOCUS THAT CONTROLS FILAMENT FORMATION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI K-12.一个控制大肠杆菌K-12中丝状形成和对辐射敏感性的基因座。
Genetics. 1964 Feb;49(2):237-46. doi: 10.1093/genetics/49.2.237.
4
REGULATORY MECHANISMS FOR SYNTHESIS OF CAPSULAR POLYSACCHARIDE IN MUCOID MUTANTS OF ESCHERICHIA COLI K12.大肠杆菌K12黏液突变体中荚膜多糖合成的调控机制
Proc Natl Acad Sci U S A. 1964 Feb;51(2):239-46. doi: 10.1073/pnas.51.2.239.
5
Selecting bacterial mutants by the penicillin method.用青霉素法筛选细菌突变体。
Science. 1960 Feb 26;131(3400):604-5. doi: 10.1126/science.131.3400.604.
6
Cell genetics and hereditary symbiosis.细胞遗传学与遗传共生
Physiol Rev. 1952 Oct;32(4):403-30. doi: 10.1152/physrev.1952.32.4.403.
7
Intracellular location and number of replicating parental DNA molecules of bacteriophages lambda and phi-X174.噬菌体λ和φ-X174复制亲代DNA分子的细胞内定位及数量
J Mol Biol. 1969 Apr 14;41(1):39-65. doi: 10.1016/0022-2836(69)90124-7.
8
Plasmid formation: a new mode of lysogeny by phase lambda.质粒形成：λ噬菌体溶原化的一种新模式。
Nature. 1969 Jul 12;223(5202):158-60. doi: 10.1038/223158a0.
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Process of infection with bacteriophage phiX174. XX. Attachment of the parental DNA of bacteriophage phiX174 to a fast-sedimenting cell component.噬菌体φX174的感染过程。XX. 噬菌体φX174的亲代DNA与快速沉降细胞成分的附着。
J Mol Biol. 1968 May 28;34(1):17-29. doi: 10.1016/0022-2836(68)90231-3.
10
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J Bacteriol. 1967 Aug;94(2):388-95. doi: 10.1128/jb.94.2.388-395.1967.
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