噬菌体λ核酸外切酶和β蛋白在基因重组中的作用。V. λ DNA的体外重组
Role of exonuclease and beta protein of phage lambda in genetic recombination. V. Recombination of lambda DNA in vitro.
作者信息
Cassuto E, Lash T, Sriprakash K S, Radding C M
出版信息
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1639-43. doi: 10.1073/pnas.68.7.1639.
Abstract
The sequential action of lambda exonuclease and polynucleotide ligase upon redundant joint molecules is sufficient to produce intact polynucleotide chains and heat-stable, biologically active molecules of lambda DNA, whereas the action of ligase alone is insufficient. These results (a) confirm the previously described mechanism of single-strand assimilation, including a subsidiary mechanism by which the further action of lambda exonuclease is arrested when a redundant strand is completely assimilated, and (b) represent a simulation of the steps in genetic recombination that follow the formation of biparental complexes (synapsis). lambda exonuclease is postulated to catalyze a concerted reaction that includes exposure of complementary sequences, formation of heteroduplex regions, and elimination of redundant branches.
摘要
λ外切核酸酶和多核苷酸连接酶对冗余连接分子的顺序作用足以产生完整的多核苷酸链以及热稳定的、具有生物活性的λ DNA分子，而仅连接酶的作用则是不够的。这些结果（a）证实了先前描述的单链同化机制，包括一种辅助机制，即当一条冗余链完全被同化时，λ外切核酸酶的进一步作用就会停止；（b）代表了对双亲复合体（联会）形成后遗传重组步骤的模拟。据推测，λ外切核酸酶催化一个协同反应，该反应包括互补序列的暴露、异源双链区域的形成以及冗余分支的消除。
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