Phondke G P, Gokhale V K, Sundaram K
Immunology. 1971 Sep;21(3):437-41.
A method which allows quantitative measurements by fluorometric assay of the binding of fluorescein-labelled antilymphocyte antibody to the antigenic receptors on the surface of lymph node cells is described. Assuming the cell surface antigens are particulate antigens with multiple reacting sites, the density of such receptors and the intrinsic association constant of the interaction have been estimated.
本文描述了一种方法,该方法可通过荧光测定法对荧光素标记的抗淋巴细胞抗体与淋巴结细胞表面抗原受体的结合进行定量测量。假设细胞表面抗原为具有多个反应位点的颗粒性抗原,已对这类受体的密度以及相互作用的固有缔合常数进行了估算。