Hammarström S, Carlsson H E, Perlmann P, Svensson S
J Exp Med. 1971 Sep 1;134(3 Pt 1):565-76. doi: 10.1084/jem.134.3.565.
Preparations of E. coli 014 lipopolysaccharide (LPS) contain a common enterobacterial antigen (CA) in large amounts or in an immunogenic form. Chemical analysis revealed, in addition to o-acetyl groups, only those sugars which are present in the basal core structure of the E. coli or Salmonella LPS (e.g., galactose, glucose, glucosamine, heptose, and ketodeoxyoctonate). On treatment with acetic acid (pH 3.2) at 100 degrees C for 1.5 hr, a fragment was liberated which after gel filtration on Sephadex G-50 appeared in the molecular weight range of 2-3 x 10(3). The fragment inhibited precipitation of alkali-treated E. coli 014 LPS by antibodies to CA from anti-E. coli 014 serum. It also inhibited hemagglutination between anti-CA antibodies and red cells coated with E. coli 08 LPS. Chemical analysis of the fragment indicated that it corresponded to the core region of E. coli 014 LPS. It contained a heptose and ketodeoxyoctonate in addition to glucose and galactose. However the fraction lacked glucosamine. Enterobacterial CA has previously been found to cross-react with colon antigen of ulcerative colitis. These results should provide a chemical basis for further studies of this cross-reactivity.
大肠杆菌014脂多糖（LPS）制剂含有大量或以免疫原性形式存在的常见肠道细菌抗原（CA）。化学分析表明，除了O-乙酰基外，仅存在于大肠杆菌或沙门氏菌LPS基础核心结构中的那些糖（例如半乳糖、葡萄糖、氨基葡萄糖、庚糖和酮脱氧辛酮酸）。在100℃用乙酸（pH 3.2）处理1.5小时后，释放出一个片段，该片段在Sephadex G-50上进行凝胶过滤后，分子量范围为2-3×10³。该片段抑制了抗大肠杆菌014血清中抗CA抗体对碱处理的大肠杆菌014 LPS的沉淀。它还抑制了抗CA抗体与包被有大肠杆菌08 LPS的红细胞之间的血凝反应。对该片段的化学分析表明，它对应于大肠杆菌014 LPS的核心区域。除了葡萄糖和半乳糖外，它还含有庚糖和酮脱氧辛酮酸。然而，该级分缺乏氨基葡萄糖。先前已发现肠道细菌CA与溃疡性结肠炎的结肠抗原发生交叉反应。这些结果应为进一步研究这种交叉反应性提供化学基础。
