肠杆菌科中一种共同抗原的分离、鉴定及生物学意义
SEPARATION, CHARACTERIZATION, AND BIOLOGICAL SIGNIFICANCE OF A COMMON ANTIGEN IN ENTEROBACTERIACEAE.
作者信息
KUNIN C M
出版信息
J Exp Med. 1963 Oct 1;118(4):565-86. doi: 10.1084/jem.118.4.565.
Abstract
An antigen common to Enterobacteriaceae and closely associated with endotoxin fractions has been separated by chromatography on DEAE cellulose employing elution with a NaCl gradient. The purified common antigen fails to coat erythrocytes, is poorly, if at all antigenic, it is non-dialyzable and excluded from sephadex G-100 gel. It is composed of polysaccharide and polypeptide. The most important property of this antigen thus far determined appears to be its interference with the specificity of the hemagglutination test commonly employed to measure antibody to O antigen of Enterobacteriaceae. It may also have taxonomic significance in classification of this family of bacteria.
摘要
一种存在于肠杆菌科细菌中且与内毒素组分密切相关的共同抗原，已通过在DEAE纤维素上进行色谱分离，并采用氯化钠梯度洗脱的方法得以分离。纯化后的共同抗原无法使红细胞致敏，其抗原性很差，即便有也微乎其微，它不可透析，并且被葡聚糖G - 100凝胶排除在外。它由多糖和多肽组成。迄今为止所确定的这种抗原的最重要特性，似乎是它会干扰通常用于检测针对肠杆菌科细菌O抗原抗体的血凝试验的特异性。它在该细菌家族的分类中可能也具有分类学意义。
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