噬菌体φX174脱氧核糖核酸小片段的基因检测
Genetic assay for small fragments of bacteriophage phi X174 deoxyribonucleic acid.
作者信息
Hutchison C A, Edgell M H
出版信息
J Virol. 1971 Aug;8(2):181-9. doi: 10.1128/JVI.8.2.181-189.1971.
Abstract
The double-stranded replicative form deoxyribonucleic acid (RF-DNA) of bacteriophage phiX174 was fragmented by pancreatic deoxyribonuclease, and the complementary strand fragments were then annealed to intact viral single strands. When such complexes infected Escherichia coli spheroplasts, some of the progeny virus bore genetic markers derived from the RF-DNA fragments. In this way, genetic markers have been salvaged from DNA fragments less than 50 nucleotides in length. This method is potentially useful as a specific assay to aid in the purification of genetically defined DNA fragments and also as a mechanism for the incorporation of small chemically synthesized DNA sequences into viral genomes.
摘要
噬菌体φX174的双链复制型脱氧核糖核酸（RF-DNA）被胰脱氧核糖核酸酶切割成片段，然后将互补链片段与完整的病毒单链退火。当这类复合物感染大肠杆菌原生质球时，一些子代病毒带有源自RF-DNA片段的遗传标记。通过这种方式，已从长度小于50个核苷酸的DNA片段中挽救了遗传标记。该方法作为一种特异性测定方法，潜在地有助于纯化基因定义的DNA片段，也可作为将小的化学合成DNA序列掺入病毒基因组的一种机制。
相似文献
1
Genetic assay for small fragments of bacteriophage phi X174 deoxyribonucleic acid.噬菌体φX174脱氧核糖核酸小片段的基因检测
J Virol. 1971 Aug;8(2):181-9. doi: 10.1128/JVI.8.2.181-189.1971.
2
Specific endonuclease R fragments of bacteriophage phiX174 deoxyribonucleic acid.噬菌体φX174脱氧核糖核酸的特异性核酸内切酶R片段
J Virol. 1972 Apr;9(4):574-82. doi: 10.1128/JVI.9.4.574-582.1972.
3
Genetic characterization of the DNA of the bacteriophage phi X174 70 S particle.噬菌体φX174 70 S颗粒DNA的遗传特性分析
Virology. 1972 May;48(2):456-62. doi: 10.1016/0042-6822(72)90056-6.
4
Location of the 5-methylcytosine group on the bacteriophage phi X174 genome.噬菌体φX174基因组上5-甲基胞嘧啶基团的位置。
J Virol. 1974 Oct;14(4):872-7. doi: 10.1128/JVI.14.4.872-877.1974.
5
Uptake of homologous single-stranded fragments by superhelical DNA: a possible mechanism for initiation of genetic recombination.超螺旋DNA对同源单链片段的摄取：遗传重组起始的一种可能机制。
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2394-8. doi: 10.1073/pnas.72.6.2394.
6
Methylation pattern of lambda deoxyribonucleic acid.λ 脱氧核糖核酸的甲基化模式
J Virol. 1972 Nov;10(5):937-42. doi: 10.1128/JVI.10.5.937-942.1972.
7
Specific fragments of phi X174 deoxyribonucleic acid produced by a restriction enzyme from Haemophilus aegyptius, endonuclease Z.由埃及嗜血杆菌的一种限制酶——核酸内切酶Z产生的φX174脱氧核糖核酸的特定片段。
J Virol. 1972 Jul;10(1):42-50. doi: 10.1128/JVI.10.1.42-50.1972.
8
PHI X174 DNA INFECTION OF LYSOZYME-INDUCED SPHEROPLASTS.溶菌酶诱导的原生质球的φX174 DNA感染
Fed Proc Transl Suppl. 1965 Jan-Feb;24:14-8.
9
Mechanism of replication of phi x174 single-stranded DNA. IX. Requirement for the Escherichia coli dnaG protein.φX174单链DNA的复制机制。IX. 对大肠杆菌dnaG蛋白的需求
J Virol. 1974 Nov;14(5):1070-5. doi: 10.1128/JVI.14.5.1070-1075.1974.
10
The structure of the DNA of bacteriophage phiX174. VI. Pyrimidine sequences in the complementary strand of the replicative form.噬菌体φX174的DNA结构。VI. 复制型互补链中的嘧啶序列
J Mol Biol. 1970 Sep 14;52(2):227-38. doi: 10.1016/0022-2836(70)90027-6.
引用本文的文献
1
Inverse PCR for Site-Directed Mutagenesis.反向 PCR 用于定点诱变。
Methods Mol Biol. 2023;2967:223-238. doi: 10.1007/978-1-0716-3358-8_18.
2
Marker rescue of adeno-associated virus (AAV) capsid mutants: a novel approach for chimeric AAV production.腺相关病毒（AAV）衣壳突变体的标志物拯救：一种生产嵌合型AAV的新方法。
J Virol. 2003 Jan;77(1):423-32. doi: 10.1128/jvi.77.1.423-432.2003.
3
Molecular genetics of vaccinia virus: demonstration of marker rescue.痘苗病毒的分子遗传学：标记拯救的证明
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1593-6. doi: 10.1073/pnas.79.5.1593.
4
Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA.使用M13衍生载体的寡核苷酸定向诱变：在任何DNA片段中产生点突变的高效通用方法。
Nucleic Acids Res. 1982 Oct 25;10(20):6487-500. doi: 10.1093/nar/10.20.6487.
5
Directed mutagenesis of DNA cloned in filamentous phage: influence of hemimethylated GATC sites on marker recovery from restriction fragments.丝状噬菌体中克隆的DNA的定向诱变：半甲基化GATC位点对从限制性片段中回收标记的影响。
Nucleic Acids Res. 1982 Oct 25;10(20):6475-85. doi: 10.1093/nar/10.20.6475.
6
Adenovirus terminal protein protects single stranded DNA from digestion by a cellular exonuclease.腺病毒末端蛋白可保护单链DNA不被细胞外切核酸酶消化。
Nucleic Acids Res. 1980 Feb 11;8(3):543-54. doi: 10.1093/nar/8.3.543.
7
Mapping of new Escherichia coli K and 15 restriction sites on specific fragments of bacteriophage phi X174.噬菌体φX174特定片段上新的大肠杆菌K和15个限制酶切位点的图谱绘制。
J Virol. 1973 Mar;11(3):378-85. doi: 10.1128/JVI.11.3.378-385.1973.
8
Specific endonuclease R fragments of bacteriophage phiX174 deoxyribonucleic acid.噬菌体φX174脱氧核糖核酸的特异性核酸内切酶R片段
J Virol. 1972 Apr;9(4):574-82. doi: 10.1128/JVI.9.4.574-582.1972.
9
Specific fragments of phi X174 deoxyribonucleic acid produced by a restriction enzyme from Haemophilus aegyptius, endonuclease Z.由埃及嗜血杆菌的一种限制酶——核酸内切酶Z产生的φX174脱氧核糖核酸的特定片段。
J Virol. 1972 Jul;10(1):42-50. doi: 10.1128/JVI.10.1.42-50.1972.
10
Formation of rings from segments of HeLa-cell nuclear deoxyribonucleic acid.由海拉细胞核脱氧核糖核酸片段形成环。
Biochem J. 1974 Dec;143(3):521-34. doi: 10.1042/bj1430521.
本文引用的文献
1
Genetic Map of Bacteriophage phiX174.噬菌体φX174的遗传图谱
J Virol. 1971 May;7(5):549-58. doi: 10.1128/JVI.7.5.549-558.1971.
2
STRUCTURE OF THE DNA OF BACTERIOPHAGE PHI-X174. V. PURINE SEQUENCES.噬菌体φ-X174的DNA结构。V. 嘌呤序列。
J Mol Biol. 1964 Aug;9:489-97. doi: 10.1016/s0022-2836(64)80221-7.
3
The structure of the DNA of bacteriophage x-174. IV. Pyrimidine sequences.噬菌体x - 174的DNA结构。IV. 嘧啶序列。
J Mol Biol. 1963 Feb;6:115-27. doi: 10.1016/s0022-2836(63)80127-8.
4
Observations on the infection of bacterial protoplasts with the deoxyribonucleic acid of bacteriophage phi X174.关于用噬菌体φX174的脱氧核糖核酸感染细菌原生质体的观察
Biochim Biophys Acta. 1963 Jun 25;72:290-7.
5
Complementary strand infectivity in phi X174 replicative form DNA.φX174复制型DNA中的互补链感染性。
J Mol Biol. 1967 Aug 14;27(3):443-51. doi: 10.1016/0022-2836(67)90050-2.
6
Separation and chain-length determination of polynucleotides by gel filtration.通过凝胶过滤法分离多核苷酸并测定其链长
Biochim Biophys Acta. 1967 May 30;138(3):466-73. doi: 10.1016/0005-2787(67)90543-6.
7
The process of infection with bacteriophage phi-X-174. XI. Infectivity of the complementary strand of the replicative form.噬菌体φ-X-174的感染过程。XI. 复制型互补链的感染性。
J Mol Biol. 1967 Feb 14;23(3):545-52. doi: 10.1016/s0022-2836(67)80124-4.
8
The process of infection with bacteriophage phi-X174. X. Mutations in a phi-X Lysis gene.噬菌体φ-X174的感染过程。X. φ-X裂解基因中的突变
J Mol Biol. 1966 Jul;18(3):429-47. doi: 10.1016/s0022-2836(66)80035-9.
9
The process of infection with bacteriophage phi-X174 VII. Ultracentrifugal analysis of the replicative form.噬菌体φ-X174的感染过程 VII. 复制型的超速离心分析
J Mol Biol. 1965 Dec;14(2):327-47. doi: 10.1016/s0022-2836(65)80185-1.
10
The process of infection with bacteriophage phi-X174. 28. Removal of the spike proteins from the phage capsid.噬菌体phi-X174的感染过程。28. 从噬菌体衣壳中去除刺突蛋白。
J Mol Biol. 1969 Jun 28;42(3):547-57. doi: 10.1016/0022-2836(69)90242-3.
Zotero 插件