Immunogenicity and specificity of collagen. XII. Demonstration by immunofluorescence and haemagglutination of antibodies with different specificity to human collagen.

A comparative investigation, using haemagglutination, haemagglutination-inhibition and immunofluorescence, was performed for demonstration of antibodies with different types of collagen specificity. In rabbit antisera to acid-soluble human collagen a fraction of species-specific antibodies to human collagen and a fraction of general, non-species-specific collagen antibodies could be demonstrated in good accordance by both techniques. Calf, rabbit, rat and guinea-pig collagen were used as heterologous control preparations.