胶原蛋白的免疫原性与特异性。VI. 使用免疫吸附技术从抗胶原蛋白血清中分离具有受限特异性的抗体组分。
Immunogenicity and specificity of collagen. VI. Separation of antibody fractions with restricted specificity from anti-collagen sera using an immunoadsorbent technique.
作者信息
Timpl R, Wolff I, Furthmayr H, Steffen C
出版信息
Immunology. 1968 Jul;15(1):145-51.
Rabbit antisera to calf collagen were investigated using columns of denatured rabbit collagen insolubilized by coupling to diazotized -aminobenzyl cellulose. Only antibodies with general collagen specificity were bound onto the immunoadsorbent. Species specific antibodies passed through the column and were isolated in the effluent. Column-bound antibodies could be desorbed by elution with collagen peptides. The separated antibody fractions exhibited in haemagglutination—inhibition experiments with calf and rabbit collagen and collagen peptides a characteristic behaviour allowing the differentiation between them. The results support the assumption drawn from serological experiments that most antisera contain mixtures of antibodies with different specificities.
利用偶联到重氮化对氨基苄基纤维素上的变性兔胶原蛋白柱，对兔抗小牛胶原蛋白抗血清进行了研究。只有具有一般胶原蛋白特异性的抗体能结合到免疫吸附剂上。种属特异性抗体则通过柱子并在流出液中分离出来。结合在柱子上的抗体可用胶原蛋白肽洗脱下来。在对小牛和兔胶原蛋白及胶原蛋白肽进行的血凝抑制实验中，分离出的抗体组分表现出一种特征性行为，能够区分它们。这些结果支持了从血清学实验得出的假设，即大多数抗血清都含有具有不同特异性的抗体混合物。
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