Timpl R, Wolff I, Furthmayr H, Steffen C
Immunology. 1968 Jul;15(1):145-51.
Rabbit antisera to calf collagen were investigated using columns of denatured rabbit collagen insolubilized by coupling to diazotized -aminobenzyl cellulose. Only antibodies with general collagen specificity were bound onto the immunoadsorbent. Species specific antibodies passed through the column and were isolated in the effluent. Column-bound antibodies could be desorbed by elution with collagen peptides. The separated antibody fractions exhibited in haemagglutination—inhibition experiments with calf and rabbit collagen and collagen peptides a characteristic behaviour allowing the differentiation between them. The results support the assumption drawn from serological experiments that most antisera contain mixtures of antibodies with different specificities.
利用偶联到重氮化对氨基苄基纤维素上的变性兔胶原蛋白柱，对兔抗小牛胶原蛋白抗血清进行了研究。只有具有一般胶原蛋白特异性的抗体能结合到免疫吸附剂上。种属特异性抗体则通过柱子并在流出液中分离出来。结合在柱子上的抗体可用胶原蛋白肽洗脱下来。在对小牛和兔胶原蛋白及胶原蛋白肽进行的血凝抑制实验中，分离出的抗体组分表现出一种特征性行为，能够区分它们。这些结果支持了从血清学实验得出的假设，即大多数抗血清都含有具有不同特异性的抗体混合物。
