Lev M, Keudell K C, Milford A F
J Bacteriol. 1971 Oct;108(1):175-8. doi: 10.1128/jb.108.1.175-178.1971.
Rumen strains of the obligate anaerobe Bacteroides melaninogenicus normally require medium supplemented with both heme and vitamin K. Sodium succinate was found to be an additional growth factor in that this compound can replace the requirement for heme in the presence of vitamin K, allowing good growth of the organism, and succinate can also partially replace the requirement for vitamin K in the presence of heme. The addition of succinate to a medium supplemented with both vitamin K and heme increases the growth rate of the culture. This ability to stimulate growth was specific for succinate, and cells grown without heme but with vitamin K and succinate were insensitive to cyanide. These experiments demonstrate a central role for succinate in the metabolism of B. melaninogenicus.
专性厌氧菌产黑素拟杆菌的瘤胃菌株通常需要添加血红素和维生素K的培养基。已发现琥珀酸钠是一种额外的生长因子，因为在有维生素K存在的情况下，该化合物可以替代对血红素的需求，使该微生物良好生长，并且在有血红素存在的情况下，琥珀酸钠也可以部分替代对维生素K的需求。向添加了维生素K和血红素的培养基中添加琥珀酸钠可提高培养物的生长速率。这种刺激生长的能力对琥珀酸钠具有特异性，并且在没有血红素但有维生素K和琥珀酸钠的情况下生长的细胞对氰化物不敏感。这些实验证明了琥珀酸钠在产黑素拟杆菌代谢中的核心作用。
