兔肌肉磷酸丙糖异构酶的活性中心。被磷酸缩水甘油标记的位点。
The active centre of rabbit muscle triose phosphate isomerase. The site that is labelled by glycidol phosphate.
作者信息
Miller J C, Waley S G
出版信息
Biochem J. 1971 Jun;123(2):163-70. doi: 10.1042/bj1230163.
Abstract
- Glycidol (2,3-epoxypropanol) phosphate is a specific irreversible inhibitor of rabbit muscle triose phosphate isomerase (EC 5.3.1.1); the site of attachment has now been studied. 2. The labelled enzyme was digested with pepsin and a modified peptide isolated. The sequence of the peptide is: Ala-Tyr-Glu-Pro-Val-Trp. 3. It is the glutamic acid residue in this peptide that is labelled: the peptide is thus a gamma-glutamyl ester derived from glycerol phosphoric acid. The same site is labelled by a mixture of glycidol and inorganic phosphate. 4. Kinetic and stereochemical features of these reactions are discussed.
摘要
- 甘油磷酸（2,3-环氧丙醇磷酸）是兔肌肉磷酸丙糖异构酶（EC 5.3.1.1）的一种特异性不可逆抑制剂；现已对其结合位点进行了研究。2. 用胃蛋白酶消化标记的酶，并分离出一种修饰肽。该肽的序列为：丙氨酸-酪氨酸-谷氨酸-脯氨酸-缬氨酸-色氨酸。3. 被标记的是该肽中的谷氨酸残基：因此该肽是衍生自甘油磷酸的γ-谷氨酰酯。相同位点也被甘油和无机磷酸盐的混合物标记。4. 讨论了这些反应的动力学和立体化学特征。
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The active centre of triose phosphate isomerase.磷酸丙糖异构酶的活性中心。
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