Kaplanis J N, Thompson M J, Dutky S R, Robbins W E
Steroids. 1979 Sep;34(3):333-45. doi: 10.1016/0039-128x(79)90084-9.
Six naturally occurring C27 ecdysteroids were isolated and identified from the tobacco hornworm during pupal-adult development five days after peak titer of molting hormone activity. In order of decreasing quantities the hormones were: 20,26-dihydroxyecdysone, 3-epi-20-hydroxyecdysone, 20hydroxyecdysone, 3-epi-20,26-dihydroxyecdysone, 3-epi-ecdysone, and ecdysone. 20-Hydroxyecdysone, in an earlier study, was the major molting hormone present at peak titer during pupal-adult development. The major ecdysteroid present during embryonic development in this insect, 26-hydroxyecdysone, was not detected. The copresence of all six of these ecdysteroids from a single developmental stage of an insect provides information on the metabolic interrelationships that exist among these steroids and on their possible function(s) in insects. The 3alpha-ecdysteroids were far less active than the 3 beta-epimers in the house fly assay. The significance of epimerization is discussed.
在蜕皮激素活性达到峰值五天后的烟草天蛾蛹 - 成虫发育阶段，六种天然存在的C27蜕皮甾体被分离并鉴定出来。按含量递减顺序，这些激素分别为：20,26 - 二羟基蜕皮酮、3 - 表 - 20 - 羟基蜕皮酮、20 - 羟基蜕皮酮、3 - 表 - 20,26 - 二羟基蜕皮酮、3 - 表 - 蜕皮酮和蜕皮酮。在早期研究中，20 - 羟基蜕皮酮是蛹 - 成虫发育阶段激素峰值时存在的主要蜕皮激素。在这种昆虫胚胎发育过程中存在的主要蜕皮甾体26 - 羟基蜕皮酮未被检测到。来自昆虫单一发育阶段的所有这六种蜕皮甾体的共存，提供了关于这些甾体之间存在的代谢相互关系以及它们在昆虫中可能功能的信息。在家蝇试验中，3α - 蜕皮甾体的活性远低于3β - 差向异构体。文中讨论了差向异构化的意义。
