Kaplanis J N, Thompson M J, Dutky S R, Robbins W E
Steroids. 1980 Sep;36(3):321-36. doi: 10.1016/0039-128x(80)90006-9.
26-Hydroxyecdysone, which is the major free recoverable ecdysteroid of older age groups of embryonated eggs of the tobacco hornworm was also the major component in 4- to 18-hour-old embryonated eggs. The other 3 beta-ecdysteroids, ecdysone, 20-hydroxyecdysone, and 20,26-dihydroxy-ecdysone, were also present and accounted for all the molting hormone activity; 26-hydroxyecdysone was devoid of molting hormone activity in the house fly assay. 20-Hydroxyecdysone was a minor component, which confirms the earlier observations that the main metabolic route for ecdysteroids during embryonic development is that leading to 26-hydroxyecdysone, whereas formation of 20-hydroxyecdysone is a minor pathway. A new 3 alpha-ecdysteroid, 3-epi-26-hydroxyecdysone, also devoid of molting hormone activity, was the second major ecdysteroid isolated from the eggs. 3-Epi-20,26-dihydroxyecdysone was detected in very minute amounts. In addition to the six 3 beta-and 3 alpha-ecdysteroids there were at least an equivalent number of unknown ecdysteroids all of which lacked molting hormone activity. Their physical properties including chromatographic behavior are discussed.
26-羟基蜕皮酮是烟草天蛾胚胎卵老龄组中主要的可游离回收的蜕皮甾体，也是4至18小时龄胚胎卵中的主要成分。其他3种β-蜕皮甾体，蜕皮酮、20-羟基蜕皮酮和20,26-二羟基蜕皮酮也存在，并占所有蜕皮激素活性；在家蝇试验中，26-羟基蜕皮酮没有蜕皮激素活性。20-羟基蜕皮酮是次要成分，这证实了早期的观察结果，即胚胎发育过程中蜕皮甾体的主要代谢途径是生成26-羟基蜕皮酮，而生成20-羟基蜕皮酮是次要途径。一种新的3α-蜕皮甾体，3-表-26-羟基蜕皮酮，也没有蜕皮激素活性，是从卵中分离出的第二种主要蜕皮甾体。检测到极少量的3-表-20,26-二羟基蜕皮酮。除了这六种3β-和3α-蜕皮甾体外，至少还有等量的未知蜕皮甾体，它们都没有蜕皮激素活性。讨论了它们的物理性质，包括色谱行为。
