Thompson M J, Svoboda J A, Weirich G F
Steroids. 1984 Mar;43(3):333-41. doi: 10.1016/0039-128x(84)90051-5.
Ecdysteroids of ovaries and newly-laid eggs (0- to 1-hour-old) of the tobacco hornworm are present mainly as conjugates (greater than 95%). Newly-laid eggs contain ecdysteroid conjugates equivalent to 21 micrograms of 26-hydroxyecdysone and 0.73 micrograms of ecdysone per gram of eggs. These levels are similar in ovaries of 93-hour-old adult females. In 1- to 18-hour-old eggs more than 63% of the ecdysteroids exist in the free form and the proportion is similar in 48- to 64-hour-old eggs. The ratio of 26-hydroxyecdysone to ecdysone in the conjugated form remains constant during oocyte maturation and embryogenesis. Though 26-hydroxyecdysone is without molting hormone activity in the house fly assay, the exceptionally high concentration of 26-hydroxyecdysone conjugate(s) in ovaries and newly-laid eggs, together with the fact that it is being released during embryogenesis, indicate some physiological role for 26-hydroxyecdysone.
烟草天蛾卵巢和新产卵（0至1小时龄）中的蜕皮甾类主要以缀合物形式存在（超过95%）。新产卵所含的蜕皮甾类缀合物相当于每克卵含21微克26 - 羟基蜕皮酮和0.73微克蜕皮酮。93小时龄成年雌蛾的卵巢中这些水平与之相似。在1至18小时龄的卵中，超过63%的蜕皮甾类以游离形式存在，48至64小时龄的卵中该比例相似。在卵母细胞成熟和胚胎发育过程中，缀合形式的26 - 羟基蜕皮酮与蜕皮酮的比例保持恒定。尽管在家蝇试验中26 - 羟基蜕皮酮没有蜕皮激素活性，但卵巢和新产卵中26 - 羟基蜕皮酮缀合物的异常高浓度，以及它在胚胎发育过程中被释放这一事实，表明26 - 羟基蜕皮酮具有某种生理作用。
