通过蛋白质合成起始因子对单个核糖体形成的控制。
Control of single ribosome formation by an initiation factor for protein synthesis.
作者信息
Kaempfer R
出版信息
Proc Natl Acad Sci U S A. 1971 Oct;68(10):2458-62. doi: 10.1073/pnas.68.10.2458.
Abstract
30 and 50S ribosomal subunits, released from polysomes upon polypeptide chain termination, possess a high affinity for each other and readily form single ribosomes. Highly purified initiation factor F3(B), acting stoichiometrically, prevents the formation of single ribosomes without promoting their dissociation. These findings are interpreted in terms of a ribosome cycle in which a limiting amount of factor F3(B) controls the number of ribosomes active in protein synthesis, in response to metabolic changes in the cell, by regulating the flow of ribosomal subunits into polysomes or into a sidetrack pool of synthetically inactive single ribosomes. The reported apparent ribosome dissociation activity of F3(B) is explained.
摘要
在多肽链终止时从多核糖体释放出来的30S和50S核糖体亚基，彼此具有很高的亲和力，并易于形成单个核糖体。高度纯化的起始因子F3(B)以化学计量方式起作用，可阻止单个核糖体的形成，而不会促进其解离。这些发现是根据核糖体循环来解释的，在该循环中，有限量的因子F3(B)通过调节核糖体亚基流入多核糖体或流入合成无活性的单个核糖体的旁路池，来响应细胞中的代谢变化，从而控制参与蛋白质合成的核糖体数量。对报道的F3(B)明显的核糖体解离活性进行了解释。
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