在起始复合物形成过程中多肽链起始因子IF-2的释放。
Release of polypeptide chain initiation factor IF-2 during initiation complex formation.
作者信息
Lockwood A H, Sarkar P, Maitra U
出版信息
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3602-5. doi: 10.1073/pnas.69.12.3602.
Abstract
Polypeptide chain initiation factor IF-2 binds to 30S ribosomal subunits. This binding is enhanced by IF-1 and IF-3. During GTP-dependent formation of a 70S initiation complex, IF-2 is released from the ribosome. During 70S initiation complex formation dependent on the methylene analogue of GTP, GMPPCH(2)P, IF-2 is not released, but remains bound to the 70S ribosome. This result suggests that IF-2 release requires GTP hydrolysis. In agreement with this presumed requirement, IF-2 functions catalytically with GTP, but stoichiometrically with GMPPCH(2)P, in bringing about 70S initiation complex formation.
摘要
多肽链起始因子IF-2与30S核糖体亚基结合。IF-1和IF-3可增强这种结合。在依赖GTP形成70S起始复合物的过程中，IF-2从核糖体上释放。在依赖GTP的亚甲基类似物GMPPCH(2)P形成70S起始复合物的过程中，IF-2不会释放，而是仍与70S核糖体结合。这一结果表明IF-2的释放需要GTP水解。与这一推测的需求一致，在促成70S起始复合物形成的过程中，IF-2与GTP起催化作用，但与GMPPCH(2)P起化学计量作用。
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