DNA复制以及与DNA结合的蛋白质。
DNA replication and proteins that bind to DNA.
作者信息
Masker W E, Eberle H
出版信息
Proc Natl Acad Sci U S A. 1971 Oct;68(10):2549-53. doi: 10.1073/pnas.68.10.2549.
Abstract
Cells of Escherichia coli 15T(-) were exposed to nalidixic acid or starved for thymine to inhibit DNA synthesis while protein synthesis was allowed to continue. These treatments are known to allow subsequent DNA synthesis to continue for many hours in the absence of protein synthesis. The proteins extracted from these cells that bind to DNA were examined by DNA-cellulose chromatography and disc-gel electrophoresis. One or two species were found to be absent, or to be present in greatly reduced amounts, under these conditions.
摘要
将大肠杆菌15T（-）细胞暴露于萘啶酸中或使其胸腺嘧啶饥饿以抑制DNA合成，同时允许蛋白质合成继续进行。已知这些处理可使后续的DNA合成在没有蛋白质合成的情况下持续数小时。通过DNA-纤维素色谱法和圆盘凝胶电泳对从这些与DNA结合的细胞中提取的蛋白质进行了检测。发现在这些条件下，有一两种蛋白质缺失或含量大幅降低。
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