萘啶酸对经甲苯处理的大肠杆菌DNA复制的影响。
Effect of nalidixic acid on DNA replication by toluene-treated Escherichia coli.
作者信息
Burger R M, Glaser D A
出版信息
Proc Natl Acad Sci U S A. 1973 Jul;70(7):1955-8. doi: 10.1073/pnas.70.7.1955.
Abstract
Nalidixic acid inhibits DNA synthesis in toluene-treated E. coli, strain B/r, as it is well known to do in vivo. Both semiconservative and repair syntheses are affected, though to different degrees. Density-transfer experiments indicate that chromosomal replication is reinitiated when nalidixic acid is removed from toluene-treated cells after exposure to the acid for one generation in vivo. For cells in vivo or after toluene-treatment, reinitiation is not seen in asynchronous cultures exposed briefly to nalidixic acid or in cells prevented from synthesizing proteins during their exposure to the acid. Reinitiation occurs at the chromosomal origin but, unlike the effect seen in vivo, replication at the old site persists.
摘要
萘啶酸抑制经甲苯处理的B/r株大肠杆菌中的DNA合成，这在体内是众所周知的。半保留合成和修复合成均受到影响，不过程度不同。密度转移实验表明，当萘啶酸在体内暴露一代后从经甲苯处理的细胞中去除时，染色体复制会重新启动。对于体内的细胞或经甲苯处理后的细胞，在短暂暴露于萘啶酸的异步培养物中或在暴露于该酸期间被阻止合成蛋白质的细胞中，看不到重新启动。重新启动发生在染色体起始点，但与体内观察到的效应不同，旧位点的复制仍会持续。
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