• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用通过区带离心分离的酵母群体来研究细胞周期。

Use of yeast populations fractionated by zonal centrifugation to study the cell cycle.

作者信息

Sebastian J, Carter B L, Halvorson H O

出版信息

J Bacteriol. 1971 Dec;108(3):1045-50. doi: 10.1128/jb.108.3.1045-1050.1971.

DOI:10.1128/jb.108.3.1045-1050.1971
PMID:4945182
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC247186/
Abstract

Zonal centrifugation in a sucrose density gradient was used to separate yeast cells primarily by size and thus by age in the cell cycle. This approach provides an alternative to synchronous growth for examining the properties of cells at different stages in the cell cycle.

摘要

在蔗糖密度梯度中进行区带离心，主要是根据酵母细胞的大小，进而根据其在细胞周期中的年龄来分离细胞。这种方法为研究细胞周期不同阶段细胞的特性提供了一种替代同步生长的方法。

相似文献

1
Use of yeast populations fractionated by zonal centrifugation to study the cell cycle.使用通过区带离心分离的酵母群体来研究细胞周期。
J Bacteriol. 1971 Dec;108(3):1045-50. doi: 10.1128/jb.108.3.1045-1050.1971.
2
Induction capacity of enzyme synthesis during the cell cycle of Saccharomyces cerevisiae.
Eur J Biochem. 1973 Sep 3;37(3):516-22. doi: 10.1111/j.1432-1033.1973.tb03013.x.
3
Synchronous and synchronized culture of Saccharomyces cerevisiae with respect to relative age of individual cells.关于单个细胞相对年龄的酿酒酵母同步培养和同步化培养。
Folia Microbiol (Praha). 1971;16(4):241-8. doi: 10.1007/BF02872805.
4
Cell cycle dependency of sporulation in Saccharomyces cerevisiae.酿酒酵母中孢子形成的细胞周期依赖性
J Bacteriol. 1972 Mar;109(3):1027-33. doi: 10.1128/jb.109.3.1027-1033.1972.
5
Derepression of mitochondria in yeast spheroplasts.酵母原生质体中线粒体的去阻遏作用。
Biochem Biophys Res Commun. 1970 Feb 6;38(3):414-22. doi: 10.1016/0006-291x(70)90729-1.
6
Cell cycle analysis.细胞周期分析
Methods Cell Biol. 1975;11:201-19.
7
Subcellular fractionation by differential and zonal centrifugation of aerobically grown glucose-de-repressed Saccharomyces carlsbergensis.通过差速离心和区带离心对需氧生长的葡萄糖去阻遏的卡尔斯伯酵母进行亚细胞分级分离。
Biochem J. 1972 Jan;126(2):381-93. doi: 10.1042/bj1260381.
8
The effect of myo-inositol on the synthesis of arginase and ornithine transaminase in baker's yeast.肌醇对面包酵母中精氨酸酶和鸟氨酸转氨酶合成的影响。
Biochim Biophys Acta. 1969 Nov 18;192(2):243-51. doi: 10.1016/0304-4165(69)90361-4.
9
Vacuolar dynamics in synchronously budding yeast.同步出芽酵母中的液泡动力学
Arch Mikrobiol. 1970;70(2):89-103. doi: 10.1007/BF00412200.
10
Overview of subcellular fractionation procedures for the yeast Saccharomyces cerevisiae.酿酒酵母亚细胞分级分离程序概述。
Curr Protoc Cell Biol. 2001 May;Chapter 3:Unit 3.7. doi: 10.1002/0471143030.cb0307s07.

引用本文的文献

1
Identification of proteins whose synthesis is modulated during the cell cycle of Saccharomyces cerevisiae.酿酒酵母细胞周期中合成受调控的蛋白质的鉴定。
Mol Cell Biol. 1982 Dec;2(12):1532-49. doi: 10.1128/mcb.2.12.1532-1549.1982.
2
Synthesis of specific identified, phosphorylated, heat shock, and heat stroke proteins through the cell cycle of Saccharomyces cerevisiae.在酿酒酵母细胞周期中特定已鉴定的、磷酸化的、热休克和中暑蛋白的合成。
Mol Cell Biol. 1982 Feb;2(2):117-26. doi: 10.1128/mcb.2.2.117-126.1982.
3
Small-sized mutants of Saccharomyces cerevisiae.酿酒酵母的小型突变体。
Genetics. 1980 Nov;96(3):561-6. doi: 10.1093/genetics/96.3.561.
4
Changes in the activity and properties of trehalase during early germination of yeast ascospores: correlation with trehalose breakdown as studied by in vivo 13C NMR.酵母子囊孢子早期萌发过程中海藻糖酶活性和性质的变化：通过体内¹³C NMR研究与海藻糖分解的相关性
Proc Natl Acad Sci U S A. 1982 Jun;79(11):3503-7. doi: 10.1073/pnas.79.11.3503.
5
Saccharomyces cerevisiae cell cycle.酿酒酵母细胞周期。
Bacteriol Rev. 1974 Jun;38(2):164-98. doi: 10.1128/br.38.2.164-198.1974.
6
Independent regulation of the nuclear RNA polymerases I and II during the yeast cell cycle.酵母细胞周期中核RNA聚合酶I和II的独立调控
Proc Natl Acad Sci U S A. 1974 Mar;71(3):769-73. doi: 10.1073/pnas.71.3.769.
7
Amino acid pools and metabolism during the cell division cycle of arginine-grown Candida utilis.
J Bacteriol. 1974 Mar;117(3):1108-16. doi: 10.1128/jb.117.3.1108-1116.1974.
8
Periodic changes in rate of amino acid uptake during yeast cell cycle.酵母细胞周期中氨基酸摄取速率的周期性变化。
J Cell Biol. 1973 Aug;58(2):401-9. doi: 10.1083/jcb.58.2.401.
9
Germination and outgrowth of single spores of Saccharomyces cerevisiae viewed by scanning electron and phase-contrast microscopy.通过扫描电子显微镜和相差显微镜观察酿酒酵母单孢子的萌发和生长。
J Bacteriol. 1972 Mar;109(3):1232-8. doi: 10.1128/jb.109.3.1232-1238.1972.
10
Cell cycle dependency of sporulation in Saccharomyces cerevisiae.酿酒酵母中孢子形成的细胞周期依赖性
J Bacteriol. 1972 Mar;109(3):1027-33. doi: 10.1128/jb.109.3.1027-1033.1972.

本文引用的文献

1
Periodic density fluctuation during the yeast cell cycle and the selection of synchronous cultures.酵母细胞周期中的周期性密度波动与同步培养物的选择。
J Bacteriol. 1970 Dec;104(3):1280-5. doi: 10.1128/jb.104.3.1280-1285.1970.
2
The nucleic acids of plant tissues; the extraction and estimation of desoxypentose nucleic acid and pentose nucleic acid.植物组织的核酸；脱氧戊糖核酸和戊糖核酸的提取与测定
Arch Biochem. 1950 Feb;25(2):262-76.
3
THE PATHWAY OF ARGININE BREAKDOWN IN SACCHAROMYCES CEREVISIAE.酿酒酵母中精氨酸分解代谢途径
Biochim Biophys Acta. 1964 Dec 9;93:650-2. doi: 10.1016/0304-4165(64)90349-6.
4
The behaviour of nucleic acids in synchronously dividing cultures of Saccharomyces cerevisiae.核酸在酿酒酵母同步分裂培养物中的行为。
Exp Cell Res. 1960 Aug;20:338-49. doi: 10.1016/0014-4827(60)90162-2.
5
The purification and properties of an alpha-glucosidase of Saccharomyces italicus Y1225.意大利酵母Y1225的α-葡萄糖苷酶的纯化及性质
Biochim Biophys Acta. 1958 Oct;30(1):28-40. doi: 10.1016/0006-3002(58)90237-3.
6
The growth of single cells. II. Saccharomyces cerevisiae.单细胞的生长。II. 酿酒酵母
Exp Cell Res. 1958 Aug;15(1):214-21. doi: 10.1016/0014-4827(58)90077-6.
7
Synchronization of cell division.细胞分裂的同步化
Bacteriol Rev. 1957 Dec;21(4):263-72. doi: 10.1128/br.21.4.263-272.1957.
8
The development of low-speed "A" series zonal rotors.低速“A”系列区带转头的研制
Natl Cancer Inst Monogr. 1966 Jun;21:113-36.
9
The use of conventional and zonal centrifugation to study the life cycle of mammalian cells. Phospholipid and macromolecular synthesis in neoplastic mast cells.利用常规离心法和区带离心法研究哺乳动物细胞的生命周期。肿瘤性肥大细胞中的磷脂和大分子合成。
Biochem J. 1970 Sep;119(3):493-9. doi: 10.1042/bj1190493.
10
Onset of DNA synthesis during the cell cycle in chemostat cultures.恒化器培养中细胞周期内DNA合成的起始
Proc Natl Acad Sci U S A. 1967 Jun;57(6):1611-7. doi: 10.1073/pnas.57.6.1611.