使用通过区带离心分离的酵母群体来研究细胞周期。
Use of yeast populations fractionated by zonal centrifugation to study the cell cycle.
作者信息
Sebastian J, Carter B L, Halvorson H O
出版信息
J Bacteriol. 1971 Dec;108(3):1045-50. doi: 10.1128/jb.108.3.1045-1050.1971.
Abstract
Zonal centrifugation in a sucrose density gradient was used to separate yeast cells primarily by size and thus by age in the cell cycle. This approach provides an alternative to synchronous growth for examining the properties of cells at different stages in the cell cycle.
摘要
在蔗糖密度梯度中进行区带离心，主要是根据酵母细胞的大小，进而根据其在细胞周期中的年龄来分离细胞。这种方法为研究细胞周期不同阶段细胞的特性提供了一种替代同步生长的方法。
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