酵母细胞周期中核RNA聚合酶I和II的独立调控
Independent regulation of the nuclear RNA polymerases I and II during the yeast cell cycle.
作者信息
Sebastian J, Takano I, Halvorson H O
出版信息
Proc Natl Acad Sci U S A. 1974 Mar;71(3):769-73. doi: 10.1073/pnas.71.3.769.
The activities of the nuclear RNA polymerases I and II have been investigated in cells of Sacharomyces cerevisiae at G(1), S, early G(2), and late G(2) stages of the cell cycle. The results show that the ratio of the activities of the two enzymes is different at these four stages, indicating that the two RNA polymerases are regulated independently during the cell cycle. The specific activity of the RNA polymerase I remains constant but that of RNA polymerase II increases sharply in cells at the beginning of the G(2) phase. These results are compatible with a pattern of continuous synthesis of the RNA polymerase I and step-wise synthesis of the RNA polymerase II during the yeast cell cycle.
已对酿酒酵母细胞在细胞周期的G(1)、S、早G(2)和晚G(2)阶段的核RNA聚合酶I和II的活性进行了研究。结果表明，这两种酶的活性比值在这四个阶段有所不同，这表明两种RNA聚合酶在细胞周期中是独立调节的。RNA聚合酶I的比活性保持恒定，但RNA聚合酶II的比活性在G(2)期开始时的细胞中急剧增加。这些结果与酵母细胞周期中RNA聚合酶I的连续合成模式和RNA聚合酶II的逐步合成模式相符。
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