Kistler W S, Lin E C
J Bacteriol. 1971 Dec;108(3):1224-34. doi: 10.1128/jb.108.3.1224-1234.1971.
In mutant cells of Escherichia coli missing the particulate l-alpha-glycerophosphate (l-alpha-GP) dehydrogenase necessary for aerobic growth on glycerol or l-alphaGP, a soluble, flavine-dependent l-alpha-GP dehydrogenase supports normal anaerobic growth rates on either of the two substrates with fumarate or nitrate as exogenous hydrogen acceptor. In an experiment in which glycerol served as the carbon source and nitrate as the acceptor, the growth of such a mutant was arrested upon the admission of air, whereas the growth of wild-type cells continued smoothly. Mutant cells lacking the soluble l-alpha-GP dehydrogenase, but possessing the particulate enzyme, can grow at normal rates aerobically on glycerol and l-alpha-GP or anaerobically on these compounds with nitrate, but not fumarate, as the hydrogen acceptor. Double mutants lacking both of the dehydrogenases fail to show significant growth on either glycerol or l-alpha-GP under any condition. Mutations affecting the anaerobic dehydrogenase (glpA locus) are situated at about minute 43 of the Taylor map, just clockwise beyond glpT, and show cotransduction with purF (1.5%), glpT (91%), and nalA (50%). The anaerobic dehydrogenase is a member of the glp regulon as judged by its inducibility by l-alpha-GP and by its constitutive formation in strains of glpR(c) genotype. The level of the anaerobic dehydrogenase is about the same in cells grown either aerobically or anaerobically with nitrate serving as a terminal hydrogen acceptor. With fumarate as terminal acceptor, the level is elevated several fold.
在大肠杆菌的突变细胞中,缺少在甘油或L-α-甘油磷酸(L-α-GP)上进行有氧生长所必需的颗粒状L-α-甘油磷酸(L-α-GP)脱氢酶,一种可溶性的、黄素依赖性的L-α-GP脱氢酶以富马酸盐或硝酸盐作为外源氢受体,支持在这两种底物中的任何一种上进行正常的厌氧生长速率。在一项以甘油作为碳源、硝酸盐作为受体的实验中,当通入空气时,这种突变体的生长就会停止,而野生型细胞的生长则继续顺利进行。缺少可溶性L-α-GP脱氢酶但拥有颗粒状酶的突变细胞,在有氧条件下可以在甘油和L-α-GP上以正常速率生长,或者在这些化合物上以硝酸盐(而非富马酸盐)作为氢受体进行厌氧生长。缺乏这两种脱氢酶的双突变体在任何条件下在甘油或L-α-GP上都无法显示出显著的生长。影响厌氧脱氢酶(glpA位点)的突变位于泰勒图谱的大约43分钟处,刚好在glpT的顺时针方向,并且显示与purF(1.5%)、glpT(91%)和nalA(50%)共转导。厌氧脱氢酶根据其被L-α-GP诱导以及在glpR(c)基因型菌株中的组成型形成来判断,是glp操纵子的一个成员。在以硝酸盐作为末端氢受体进行有氧或厌氧生长的细胞中,厌氧脱氢酶的水平大致相同。以富马酸盐作为末端受体时,其水平会升高几倍。