Ruch F E, Lengeler J, Lin E C
J Bacteriol. 1974 Jul;119(1):50-6. doi: 10.1128/jb.119.1.50-56.1974.
The utilization of glycerol as a carbon source for growth by Klebsiella aerogenes, strain 2103, involves separate aerobic (sn-glycerol-3-phosphate or G3P) and anaerobic (dihydroxyacetone or DHA) pathways of catabolism. Enzyme and transport activities of the aerobic pathway are elevated in cells grown under oxygenated conditions on glycerol or G3P. Anaerobic growth on G3P as carbon source requires the presence of an exogenous hydrogen acceptor such as fumarate; cells thus grown also are highly induced in the G3P pathway. Anaerobic growth on glycerol requires no exogenous hydrogen acceptors; cells thus grown are highly induced in the DHA pathway but almost uninduced in the G3P pathway and the addition of fumarate electron acceptors has no effect on the relative levels of the two pathways. When both glycerol and G3P are provided anaerobically with fumarate, the DHA pathway is still preferentially induced, which probably accounts for the exclusive utilization of glycerol until its exhaustion. These observations suggest the presence of a regulatory control of G3P pathway imposed by the operation of the DHA pathway.
产气克雷伯菌2103菌株利用甘油作为碳源进行生长，涉及单独的有氧（sn-甘油-3-磷酸或G3P）和厌氧（二羟基丙酮或DHA）分解代谢途径。在含氧条件下于甘油或G3P上生长的细胞中，有氧途径的酶活性和转运活性会升高。以G3P作为碳源进行厌氧生长需要存在外源性氢受体，如富马酸；如此生长的细胞在G3P途径中也会被高度诱导。以甘油进行厌氧生长不需要外源性氢受体；如此生长的细胞在DHA途径中被高度诱导，但在G3P途径中几乎不被诱导，并且添加富马酸电子受体对两条途径的相对水平没有影响。当甘油和G3P都在厌氧条件下与富马酸一起提供时，DHA途径仍然优先被诱导，这可能解释了在甘油耗尽之前对其的唯一利用。这些观察结果表明，DHA途径的运作对G3P途径存在调节控制。
