β-半乳糖苷酶催化的β-D-吡喃半乳糖叠氮化物的水解。

Biochem J. 1971 Dec;125(3):717-9. doi: 10.1042/bj1250717.

beta-d-Galactopyranosyl azide is hydrolysed by the beta-galactosidase of Escherichia coli to galactose and azide ion at a mechanistically significant rate. 2. Methyl 1-thio-beta-d-galactopyranoside is a competitive inhibitor of the hydrolysis of the azide and of o-nitrophenyl beta-d-galactopyranoside with K(i) 1.8mm. 3. beta-Galactosidase can thus hydrolyse a range of substrates of general structure beta-d-galactopyranosyl-X(Y), where the atom X has a lone pair of electrons on which the enzyme may act as a Lewis or Brønsted acid, but in which the length of the bond cleaved varies significantly, which is inconsistent with the orbital steering hypothesis.

β -D-吡喃半乳糖叠氮化物被大肠杆菌的β-半乳糖苷酶以具有重要机制意义的速率水解为半乳糖和叠氮离子。2. 1-硫代-β -D-吡喃半乳糖苷甲基酯是叠氮化物和邻硝基苯基-β -D-吡喃半乳糖苷水解的竞争性抑制剂，抑制常数（Ki）为1.8毫摩尔。3. 因此，β-半乳糖苷酶可以水解一系列具有通式β -D-吡喃半乳糖基-X(Y)的底物，其中原子X具有孤对电子，酶可作为路易斯酸或布朗斯特酸作用于该孤对电子，但所断裂键的长度有显著差异，这与轨道定向假说不一致。

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Biochem J. 1971 Dec;125(3):717-9. doi: 10.1042/bj1250717.

beta-d-Galactopyranosyl azide is hydrolysed by the beta-galactosidase of Escherichia coli to galactose and azide ion at a mechanistically significant rate. 2. Methyl 1-thio-beta-d-galactopyranoside is a competitive inhibitor of the hydrolysis of the azide and of o-nitrophenyl beta-d-galactopyranoside with K(i) 1.8mm. 3. beta-Galactosidase can thus hydrolyse a range of substrates of general structure beta-d-galactopyranosyl-X(Y), where the atom X has a lone pair of electrons on which the enzyme may act as a Lewis or Brønsted acid, but in which the length of the bond cleaved varies significantly, which is inconsistent with the orbital steering hypothesis.

β -D-吡喃半乳糖叠氮化物被大肠杆菌的β-半乳糖苷酶以具有重要机制意义的速率水解为半乳糖和叠氮离子。2. 1-硫代-β -D-吡喃半乳糖苷甲基酯是叠氮化物和邻硝基苯基-β -D-吡喃半乳糖苷水解的竞争性抑制剂，抑制常数（Ki）为1.8毫摩尔。3. 因此，β-半乳糖苷酶可以水解一系列具有通式β -D-吡喃半乳糖基-X(Y)的底物，其中原子X具有孤对电子，酶可作为路易斯酸或布朗斯特酸作用于该孤对电子，但所断裂键的长度有显著差异，这与轨道定向假说不一致。