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精液中遗传性酶多态性的检测(作者译)

[The detection of inherited enzyme polymorphism in semen (author's transl)].

作者信息

Schwerd W, Fehrer H D

出版信息

Z Rechtsmed. 1979 Jul 17;83(2):129-38. doi: 10.1007/BF02092269.

Abstract

Our investigation of the occurrence of the enzymes phosphoglucomutase (PGM), glutamate-pyruvate-transaminase (GPT), adenylatkinase (AK), adenosine-desaminase (ADA), and 6-phophogluconate-dehydrogenase (6-PGD) produced the following results: The phosphoglucomutase type was demonstrated in the most sperm samples and seminal stains in accordance with the corresponding blood type. This enzyme is rather stable and could still be demonstrated well in 1-month old stains. The glutamate-pyruvate-transaminase can only seldom be determined in semen and seminal stains. We only found the GPT 1 type, which is known to have usually the strongest activity. The adenylatekinase was demonstrable in the most fresh ejaculates (not older than 24 h) and in about half the seminal stains (not older than 7 days)--The AK--2-band gets weak with increasing lay days, which may lead to incorrect determinations. The adenosine-desaminase could not be determined in sperm. On the contrary, 6-phosphogluconate-dehydrogenase could be demonstrated in fresh semen samples and also partly in seminal stains up to 7 days. The demonstration of the enzymes did not depend in any system on the secretor type.

摘要

我们对磷酸葡萄糖变位酶(PGM)、谷丙转氨酶(GPT)、腺苷酸激酶(AK)、腺苷脱氨酶(ADA)和6-磷酸葡萄糖酸脱氢酶(6-PGD)等酶的出现情况进行了调查,结果如下:在大多数精子样本和精液污渍中都检测到了与相应血型一致的磷酸葡萄糖变位酶类型。这种酶相当稳定,在1个月大的污渍中仍能很好地检测到。谷丙转氨酶在精液和精液污渍中很少能检测到。我们只发现了GPT 1型,已知其通常具有最强的活性。腺苷酸激酶在大多数新鲜射精样本(不超过24小时)和约一半的精液污渍(不超过7天)中可以检测到——随着放置天数的增加,AK-2带会变弱,这可能导致错误的检测结果。精子中无法检测到腺苷脱氨酶。相反,6-磷酸葡萄糖酸脱氢酶可以在新鲜精液样本中检测到,在长达7天的精液污渍中也能部分检测到。酶的检测在任何系统中都不依赖于分泌型。

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