Aderjan R, Doster S, Petri H, Schmidt G
Z Rechtsmed. 1979 Aug;83(3):201-7. doi: 10.1007/BF02333322.
The recovery measurements in rat tissues performed via i.p. injected radioactive digoxin derivates (3H-digoxin, 125J-digoxin derivative) showed that approximately 50% of the total glycoside content will be extracted. Thus, an addition of digoxin standards to drug-free tissues may lead to false negative determinations. By comparison of the radioactivity before and after extraction the following results were obtained: Recovery from tissues 3H-digoxin 50% 125J-digoxin 40% from serum 3H-digoxin 60% added to drug free tissue homogenates 3H-digoxin 85% After i.p. application of 15 mg/kg of beta-methyldigoxin to BD9 (Berlin)-rats the resulting tissue concentrations were extracted by Amberlite XAD-2. beta-Methyldigoxin and its metabolites digoxin and digoxinbisdigitoxide could be separated and distinguished from artifacts by fluorescence detection on HPTLC-plates with a detection limit of 60 ng/spot. Concentration determined by radioimmunoassay are in satisfactory agreement with HPTLC results.
通过腹腔注射放射性地高辛衍生物（3H-地高辛、125I-地高辛衍生物）对大鼠组织进行的回收率测量表明，约50%的总糖苷含量会被提取出来。因此，向无药物组织中添加地高辛标准品可能会导致假阴性测定。通过比较提取前后的放射性，得到以下结果：组织中3H-地高辛的回收率为50%，125I-地高辛为40%；血清中3H-地高辛的回收率为60%；添加到无药物组织匀浆中的3H-地高辛回收率为85%。对BD9（柏林）大鼠腹腔注射15mg/kg的β-甲基地高辛后，用Amberlite XAD-2提取所得的组织浓度。β-甲基地高辛及其代谢产物地高辛和地高辛双洋地黄毒苷可通过在高效薄层层析板上进行荧光检测与假象分离并区分，检测限为60ng/斑点。放射免疫测定法测定的浓度与高效薄层层析结果吻合良好。
