弹性蛋白酶的特异性：猪胰弹性蛋白酶II和犬白细胞弹性蛋白酶对胰岛素氧化β链的降解作用
Specificity of elastases: degradation of the oxidized beta-chain of insulin by porcine pancreatic elastase II and dog leucocyte elastase.
作者信息
Ardelt W, Tomczak Z, Dudek-Wojciechowska G
出版信息
Acta Biochim Pol. 1979;26(3):267-73.
Porcine elastase II (EC 3.4.21.-), a pancreatic proteinase with elastolytic activity, hydrolyses the oxidized beta-chain of insulin with major cleavages occurring at Leu17-Val18, Phe24-Phe25, Phe25-Tyr26 and Tyr26-Thr27. Canine leucocytic elastase splits the same substrate with major sites at Val12-Glu13 and Val18-Cys19 O3H. This indicates similarity of elastase II to chymotrypsins (EC 3.4.21.1 or 3.4.21.2) and of dog leucocyte enzyme to human granulocyte elastase and porcine pancreatic elastase I (EC 3.4.21.11).
猪弹性蛋白酶II（EC 3.4.21.-）是一种具有弹性水解活性的胰腺蛋白酶，它能水解胰岛素的氧化β链，主要裂解位点发生在Leu17-Val18、Phe24-Phe25、Phe25-Tyr26和Tyr26-Thr27处。犬白细胞弹性蛋白酶裂解相同底物时的主要位点在Val12-Glu13和Val18-Cys19 O3H处。这表明弹性蛋白酶II与胰凝乳蛋白酶（EC 3.4.21.1或3.4.21.2）具有相似性，犬白细胞酶与人类粒细胞弹性蛋白酶和猪胰腺弹性蛋白酶I（EC 3.4.21.11）具有相似性。
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