一种基于反渗透和硫酸铵沉淀法的、用于纯化人尿激肽释放酶的简单大规模工艺。

A simple, large scale process for purifying human urinary kallikrein, based on reverse osmosis and ammonium sulfate precipitation.

作者信息

Figueiredo A F, Mares-Guia M

出版信息

Adv Exp Med Biol. 1979;120A:305-12. doi: 10.1007/978-1-4757-0926-1_29.

Abstract

A simple, large scale process for the purification of human urinary kallikrein is described which is based upon concentration by reverse osmosis, ammonium sulfate precipitation, and gel filtration on a column of Sephadex-G-150. The yield in protein is higher than any reported in the literature to this date; the purified enzyme seems to be identical to that reported by Figueiredo and Mares-Guia at the Kinin-Symposium in Paris, 1978, based on the procedure of Hial, et al., (1974).

摘要

本文描述了一种简单的大规模纯化人尿激肽释放酶的方法，该方法基于反渗透浓缩、硫酸铵沉淀以及在Sephadex-G-150柱上进行凝胶过滤。蛋白质产量高于迄今为止文献报道的任何产量；基于Hial等人（1974年）的方法，纯化后的酶似乎与1978年在巴黎激肽研讨会上Figueiredo和Mares-Guia报道的酶相同。