Romero E M, Brenner R R
J Bacteriol. 1966 Jan;91(1):183-8. doi: 10.1128/jb.91.1.183-188.1966.
Romero, Ethel M. (Universidad Nacional de la Plata, La Plata, Argentina), and Rodolfo M. Brenner. Fatty acids synthesized from hexadecane by Pseudomonas aeruginosa. J. Bacteriol. 91:183-188. 1966.-The lipids extracted from Pseudomonas aeruginosa incubated with hexadecane in a mineral medium were separated into a nonpolar and three polar fractions by thin-layer chromatography. The fatty acid composition of the four cellular fractions and that of the lipids excreted into the medium was studied by gas-liquid chromatography. Saturated fatty acids with 14 to 22 carbons were recognized, together with monoenoic, dienoic, and hydroxylated acids. Hydroxylated fatty acids were principally found in two polar fractions containing rhamnose and glucose; the other polar fraction, containing serine, alanine, ethanolamine, and leucine, was richer in monoenoic fatty acids. Octadecadienoic acid was found in the neutral fraction.
罗梅罗，埃塞尔·M.（阿根廷拉普拉塔国立大学，拉普拉塔），以及鲁道夫·M. 布伦纳。铜绿假单胞菌由十六烷合成的脂肪酸。《细菌学杂志》91:183 - 188。1966年。——在矿物培养基中与十六烷一起培养的铜绿假单胞菌所提取的脂质，通过薄层色谱法被分离为一个非极性部分和三个极性部分。通过气液色谱法研究了四个细胞部分以及分泌到培养基中的脂质的脂肪酸组成。识别出了含14至22个碳的饱和脂肪酸，以及单烯酸、二烯酸和羟基化酸。羟基化脂肪酸主要存在于含有鼠李糖和葡萄糖的两个极性部分中；另一个含有丝氨酸、丙氨酸、乙醇胺和亮氨酸的极性部分则富含单烯脂肪酸。十八碳二烯酸存在于中性部分中。
