Nester E W, Jensen R A
J Bacteriol. 1966 Apr;91(4):1594-8. doi: 10.1128/jb.91.4.1594-1598.1966.
Nester, E. W. (University of Washington, Seattle), and R. A. Jensen. Control of aromatic acid biosynthesis in Bacillus subtilis: sequential feedback inhibition. J. Bacteriol. 91:1594-1598. 1966.-The three major end products of aromatic acid synthesis, tyrosine, phenylalanine, and tryptophan, were tested for their ability to inhibit the first enzymes of the three terminal branches of the pathway as well as the enzyme common to both tyrosine and phenylalanine synthesis. Tyrosine inhibits the activity of prephenate dehydrogenase and also prephenate dehydratase to a limited extent. Phenylalanine inhibits the activity of prephenate dehydratase and, at much higher concentrations, prephenate dehydrogenase. Tryptophan inhibits the activity of anthranilate synthetase and, to some extent, prephenate dehydrogenase and prephenate dehydratase. Chorismate mutase is not inhibited by either 1 mm tyrosine or 1 mm phenylalanine when these are present singly or together in the reaction mixture. The significance of the feedback control of the terminal branches to the feedback control of that part of the pathway common to the synthesis of all three amino acids is discussed.
内斯特，E.W.（华盛顿大学，西雅图）和R.A.詹森。枯草芽孢杆菌中芳香酸生物合成的控制：顺序反馈抑制。《细菌学杂志》91:1594 - 1598。1966年。——对芳香酸合成的三种主要终产物酪氨酸、苯丙氨酸和色氨酸进行了测试，以考察它们抑制该途径三个末端分支的第一种酶以及酪氨酸和苯丙氨酸合成共同的酶的能力。酪氨酸抑制预苯酸脱氢酶的活性，也在一定程度上抑制预苯酸脱水酶的活性。苯丙氨酸抑制预苯酸脱水酶的活性，在高得多的浓度下也抑制预苯酸脱氢酶的活性。色氨酸抑制邻氨基苯甲酸合成酶的活性，在一定程度上也抑制预苯酸脱氢酶和预苯酸脱水酶的活性。当反应混合物中单独或同时存在1毫摩尔酪氨酸或1毫摩尔苯丙氨酸时，分支酸变位酶不受其抑制。讨论了末端分支的反馈控制对所有三种氨基酸合成共同的途径部分的反馈控制的意义。
