枯草芽孢杆菌孢子核糖体的分离与特性分析。
Isolation and characterization of ribosomes from Bacillus subtilis spores.
作者信息
Bishop H L, Doi R H
出版信息
J Bacteriol. 1966 Feb;91(2):695-701. doi: 10.1128/jb.91.2.695-701.1966.
Abstract
Bishop, Helen L. (Syracuse University, Syracuse, N.Y.), and Roy H. Doi. Isolation and characterization of ribosomes from Bacillus subtilis spores. J. Bacteriol. 91:695-701. 1966.-The isolation of ribosomes from Bacillus subtilis spores was accomplished by freezing the spores in liquid nitrogen and grinding the spore pellet with an equal weight of levigated alumina. The ribosomes, which were adsorbed to the alumina, were freed by the addition of vegetative-cell ribosomes or bulk ribonucleic acid (RNA) to the crude alumina-ground extract. The spore ribosomes had sedimentation properties and RNA and protein compositions similar to those of vegetative-cell ribosomes. The difficulty encountered in obtaining spore ribosomes by ordinary extraction methods may be the result of nuclease and protease activities which were demonstrated in spore extracts.
摘要
毕晓普，海伦·L.（纽约州锡拉丘兹市锡拉丘兹大学）和罗伊·H.多伊。枯草芽孢杆菌孢子核糖体的分离与特性研究。《细菌学杂志》91:695 - 701。1966年。——从枯草芽孢杆菌孢子中分离核糖体的方法是，先将孢子在液氮中冷冻，然后用等重的研磨过的氧化铝研磨孢子沉淀。吸附在氧化铝上的核糖体，通过向粗制的氧化铝研磨提取物中加入营养细胞核糖体或大量核糖核酸（RNA）而被释放出来。孢子核糖体的沉降特性以及RNA和蛋白质组成与营养细胞核糖体相似。用普通提取方法获取孢子核糖体时遇到的困难，可能是由于在孢子提取物中证实存在核酸酶和蛋白酶活性。
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