酒精对产气荚膜梭菌孢子的激活与损伤
Activation and injury of Clostridium perfringens spores by alcohols.
作者信息
Craven S E, Blankenship L C
出版信息
Appl Environ Microbiol. 1985 Aug;50(2):249-56. doi: 10.1128/aem.50.2.249-256.1985.
Abstract
The activation properties of Clostridium perfringens NCTC 8679 spores were demonstrated by increases in CFU after heating in water or aqueous alcohols. The temperature range for maximum activation, which was 70 to 80 degrees C in water, was lowered by the addition of alcohols. The response at a given temperature was dependent on the time of exposure and the alcohol concentration. The monohydric alcohols and some, but not all, of the polyhydric alcohols could activate spores at 37 degrees C. The concentration of a monohydric alcohol that produced optimal spore activation was inversely related to its lipophilic character. Spore injury, which was manifested as a dependence on lysozyme for germination and colony formation, occurred under some conditions of alcohol treatment that exceeded those for optimal spore activation. Treatment with aqueous solutions of monohydric alcohols effectively activated C. perfringens spores and suggests a hydrophobic site for spore activation.
摘要
产气荚膜梭菌NCTC 8679芽孢的活化特性通过在水或醇水溶液中加热后CFU的增加得以证明。在水中最大活化的温度范围为70至80摄氏度，添加醇类可降低该温度范围。给定温度下的反应取决于暴露时间和醇浓度。一元醇和部分（但不是全部）多元醇可在37摄氏度下活化芽孢。产生最佳芽孢活化效果的一元醇浓度与其亲脂性呈负相关。在某些超过最佳芽孢活化条件的醇处理条件下，会发生芽孢损伤，表现为芽孢萌发和菌落形成对溶菌酶的依赖性。用一元醇水溶液处理可有效活化产气荚膜梭菌芽孢，并提示存在一个芽孢活化的疏水位点。
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