Emerson S U, Tokuyasu K, Simon M I
Science. 1970 Jul 10;169(3941):190-2. doi: 10.1126/science.169.3941.190.
Newly synthesized regions of Bacillus subtilis flagella were labeled with fluorophenylalanine or [(3)H]leucine. The flagella were then examined for altered gross morphology or by radioautography. Results of both experiments indicate that flagella elongate in vivo by polymerization of flagellin subunits onto the distal end of the filament.
用氟苯丙氨酸或[³H]亮氨酸标记枯草芽孢杆菌鞭毛的新合成区域。然后通过放射自显影或检查鞭毛的总体形态变化来进行观察。两个实验的结果均表明,鞭毛在体内通过鞭毛蛋白亚基聚合到丝状体的远端而延长。