Yotis W, Stanke R
J Bacteriol. 1966 Nov;92(5):1285-9. doi: 10.1128/jb.92.5.1285-1289.1966.
Yotis, William (Loyola University, Hines, Ill.), and Ronald Stanke. Bacteriostatic action of progesterone on staphylococci and other microorganisms. J. Bacteriol. 92:1285-1289. 1966.-Progesterone has been examined in vitro for antibacterial activity against 10 microorganisms. Turbidimetric and manometric techniques were used to assay the antibacterial activity of progesterone. The organisms tested consisted of Staphylococcus aureus, S. epidermidis, Gaffkya tetragena, Bacillus subtilis, Listeria monocytogenes, Candida albicans, Escherichia coli, Aerobacter aerogenes, Salmonella paratyphi, and Proteus vulgaris. Antibacterial action was shown by progesterone only against the gram-positive microorganisms when they were grown in tryptic soy broth containing 10 to 20 mug of progesterone per ml. Pregnenolone, 4-pregnen-20beta-ol-3-one, and 5alpha-pregnane also possessed antistaphylococcal properties, whereas pregnanolone, pregnandione, 11alpha-hydroxyprogesterone, and 17alpha-hydroxyprogesterone did not. The bacteriostatic action of progesterone on staphylococci was exerted primarily during the first 8 hr of incubation, and it was reduced in the presence of oxygen. In the presence of 20 mug of progesterone per ml, there was significant reduction in the oxidation by resting staphylococcal suspensions or utilization by staphylococci of pyruvate as an energy source during growth.
约蒂斯，威廉（伊利诺伊州欣斯市洛约拉大学），以及罗纳德·斯坦克。孕酮对葡萄球菌及其他微生物的抑菌作用。《细菌学杂志》92:1285 - 1289。1966年。——已在体外检测了孕酮对10种微生物的抗菌活性。采用比浊法和测压技术测定孕酮的抗菌活性。所检测的微生物包括金黄色葡萄球菌、表皮葡萄球菌、四联加夫基菌、枯草芽孢杆菌、单核细胞增生李斯特菌、白色念珠菌、大肠杆菌、产气气杆菌、副伤寒沙门菌和普通变形杆菌。当革兰氏阳性微生物在每毫升含10至20微克孕酮的胰蛋白胨大豆肉汤中生长时，仅孕酮对其显示出抗菌作用。孕烯醇酮、4 - 孕烯 - 20β - 醇 - 3 - 酮和5α - 孕烷也具有抗葡萄球菌特性，而孕烷醇酮、孕二酮、11α - 羟基孕酮和17α - 羟基孕酮则没有。孕酮对葡萄球菌的抑菌作用主要在培养的最初8小时发挥，且在有氧存在时作用减弱。在每毫升含20微克孕酮的情况下，静止的葡萄球菌悬液的氧化作用或葡萄球菌在生长过程中利用丙酮酸作为能源的能力均显著降低。
