YOTIS W W
J Bacteriol. 1963 Apr;85(4):911-7. doi: 10.1128/jb.85.4.911-917.1963.
Yotis, William W. (Loyola University, Chicago, Ill.). Absorption of the antibacterial serum factor by staphylococci. J. Bacteriol. 85:911-917. 1963.-The absorption of antibacterial serum factor by eight bacterial species and one yeast showed good correlation with the sensitivity of the organisms to the serum factor. The serum factor was removed from aqueous solution by coagulase-positive and coagulase-negative strains of staphylococci and Bacillus subtilis, and the oxygen consumption of the bacteria was inhibited by prior exposure to the serum factor. Escherichia coli, Neisseria catarrhalis, Proteus vulgaris, Bacillus megaterium, Mycobacterium phlei, and Saccharomyces cerevisiae failed to absorb the serum factor, and their respiration was not inhibited by prior exposure to 40 mg or more of the serum factor per ml. Staphylococci treated with 0.25 mg per ml of coagulase were almost completely refractory to the antibacterial action of 2 mg of serum factor per ml, and the serum factor was not absorbed. When the staphylococci were first treated with the serum factor, subsequent treatment with coagulase had no effect. Exposure of staphylocci to heat (70 C for 1 hr), 3.6% formaldehyde, 1 n sodium hydroxide, and 1 n hydrochloric acid did not prevent absorption of the serum factor. However, pretreatment with 88% liquefied phenol partially prevented serum factor absorption. The absorption and antibacterial activity of the serum factor were dependent on the concentration, the time and temperature of exposure, and the nature and concentration of salts present.
约蒂斯，威廉·W.（伊利诺伊州芝加哥洛约拉大学）。葡萄球菌对抗菌血清因子的吸收。《细菌学杂志》85:911 - 917。1963年。——8种细菌和1种酵母对抗菌血清因子的吸收与这些微生物对血清因子的敏感性具有良好的相关性。凝固酶阳性和阴性的葡萄球菌菌株以及枯草芽孢杆菌可从水溶液中去除血清因子，并且细菌的耗氧量会因预先接触血清因子而受到抑制。大肠杆菌、卡他莫拉菌、普通变形杆菌、巨大芽孢杆菌、草分枝杆菌和酿酒酵母无法吸收血清因子，并且预先接触每毫升40毫克或更多的血清因子不会抑制它们的呼吸作用。用每毫升0.25毫克凝固酶处理的葡萄球菌对每毫升2毫克血清因子的抗菌作用几乎完全具有抗性，并且血清因子未被吸收。当葡萄球菌首先用血清因子处理时，随后用凝固酶处理没有效果。将葡萄球菌暴露于热（70℃1小时）、3.6%甲醛、1N氢氧化钠和1N盐酸中不会阻止血清因子的吸收。然而，用88%液化苯酚预处理可部分阻止血清因子的吸收。血清因子的吸收和抗菌活性取决于其浓度、暴露的时间和温度以及存在的盐的性质和浓度。
