Henis Y, Gould J R, Alexander M
Appl Microbiol. 1966 Jul;14(4):513-24. doi: 10.1128/am.14.4.513-524.1966.
Ether extracts of cultures of 29 strains representing 6 species of Bacillus, and of individual strains of Escherichia coli, Aerobacter aerogenes, and Pseudomonas aeruginosa were examined in a gas chromatograph by use of flame ionization and electron capture detectors. Among the products detected were compounds with the chromatographic characteristics of acetic, propionic, and butyric acids, ethyl alcohol, diacetyl, acetoin, and 2,3-butanediol. The differences in peak areas of the various products formed by the bacteria were determined statistically for the chromatograms obtained with the two detectors, and the peaks were arranged in order of decreasing areas to yield a signature for each bacterial strain. Different signatures were obtained for the various genera and species and for strains of the same species. B. licheniformis, B. subtilis, and A. aerogenes formed significant quantities of a number of volatile compounds, and qualitative and quantitative differences between strains were noted. The electron capture detector was particularly sensitive to diacetyl and acetoin as well as to unknown compounds. By use of this detector, the presence of 5 pg of diacetyl and 20 pg of acetoin could be demonstrated. The quantity of acetoin detected in B. subtilis and B. licheniformis cultures was present in as little as 6.3 x 10(-3) muliters of medium.
对代表芽孢杆菌属6个种的29个菌株以及大肠杆菌、产气气杆菌和铜绿假单胞菌的单个菌株的培养物的乙醚提取物，使用火焰离子化检测器和电子捕获检测器在气相色谱仪中进行了检测。检测到的产物中有具有乙酸、丙酸和丁酸、乙醇、双乙酰、乙偶姻和2,3 - 丁二醇色谱特征的化合物。对用这两种检测器获得的色谱图，对细菌形成的各种产物的峰面积差异进行了统计学测定，并将峰按面积递减顺序排列，以产生每个细菌菌株的特征图谱。不同的属、种以及同种的不同菌株都获得了不同的特征图谱。地衣芽孢杆菌、枯草芽孢杆菌和产气气杆菌形成了大量的挥发性化合物，并且注意到菌株之间存在定性和定量差异。电子捕获检测器对双乙酰、乙偶姻以及未知化合物特别敏感。使用该检测器，可以检测到5皮克的双乙酰和20皮克的乙偶姻。在枯草芽孢杆菌和地衣芽孢杆菌培养物中检测到的乙偶姻量，在低至6.3×10(-3)微升的培养基中就已存在。
