Weirether F J, Lewis E E, Rosenwald A J, Lincoln R E
Appl Microbiol. 1966 Mar;14(2):284-91. doi: 10.1128/am.14.2.284-291.1966.
A simple, rapid method, based on the Oudin single diffusion technique, is described for the quantitative assay of staphylococcal enterotoxin B. The method yields reproducible results without close control of such assay variables as temperature, antiserum dilution, and assay time, provided that the ionic strength is maintained above 0.2 n sodium chloride equivalent.
本文描述了一种基于奥丁单扩散技术的简单、快速方法,用于定量检测葡萄球菌肠毒素B。只要离子强度保持在高于0.2N氯化钠当量的水平,该方法无需严格控制诸如温度、抗血清稀释度和检测时间等检测变量,就能产生可重复的结果。