Mah R A, Fung D Y, Morse S A
Appl Microbiol. 1967 Jul;15(4):866-70. doi: 10.1128/am.15.4.866-870.1967.
A synthetic medium was devised for growth of Staphylococcus aureus strain S-6. The growth yield in synthetic medium was compared to that in complex medium containing an equivalent amount of protein hydrolysate. Enterotoxin B formation in the two media was also compared. The defined medium was composed of inorganic salts, 11 amino acids (glycine, valine, leucine, threonine, phenylalanine, tyrosine, cysteine, methionine, proline, arginine, and histidine), and three vitamins (thiamine, nicotinic acid, and biotin). Biotin was a growth factor requirement of S-6 when glutamic acid but not glucose was used as a carbon source. The quantity of enterotoxin B produced in the defined medium was about one-seventh of that produced in complex medium, even though the growth yields were similar.
设计了一种用于金黄色葡萄球菌菌株S-6生长的合成培养基。将合成培养基中的生长产量与含有等量蛋白水解物的复合培养基中的生长产量进行比较。还比较了两种培养基中肠毒素B的形成。限定培养基由无机盐、11种氨基酸(甘氨酸、缬氨酸、亮氨酸、苏氨酸、苯丙氨酸、酪氨酸、半胱氨酸、蛋氨酸、脯氨酸、精氨酸和组氨酸)和三种维生素(硫胺素、烟酸和生物素)组成。当使用谷氨酸而非葡萄糖作为碳源时,生物素是S-6生长所需的一种生长因子。尽管生长产量相似,但限定培养基中产生的肠毒素B的量约为复合培养基中产生量的七分之一。