Rozee K R, Leers W D
Can Med Assoc J. 1967 Mar 11;96(10):597-9.
The hemagglutinin of all three types of reovirus was inactivated by chloroform with little or no diminution in virus infectivity. Albumin and other proteins protected the hemagglutinin from inactivation by chloroform. When crude reovirus suspensions were centrifuged in a sucrose density gradient, two hemagglutinin fractions were collected. The fraction which sedimented more rapidly contained the bulk of infectious virus, but both lost hemagglutinin activity when extracted with chloroform. Ether extraction of the fractions did not reduce either the infectivity or hemagglutinin titre.
所有三种呼肠孤病毒的血凝素都能被氯仿灭活，而病毒感染力几乎没有或仅有轻微下降。白蛋白和其他蛋白质可保护血凝素不被氯仿灭活。当粗制呼肠孤病毒悬液在蔗糖密度梯度中离心时，可收集到两个血凝素组分。沉降较快的组分含有大部分感染性病毒，但用氯仿提取时，两者都失去了血凝素活性。用乙醚提取这些组分不会降低其感染力或血凝素滴度。
