Fontana A, Weiner H L
J Immunol. 1980 Dec;125(6):2660-4.
In studying reovirus interactions with lymphocytes, we have found that reovirus type 3, but not type 1, inhibits the in vitro proliferative response of murine splenic lymphocytes to concanavalin A (Con A). By analyzing recombinant clones containing genes from both reovirus types 1 and 3, we found that the S1 gene, the gene that encodes the viral hemagglutinin, is responsible for the inhibitory effect. In addition we found that type 3, but not type 1, generates suppressor T cells in vitro capable of suppressing Con A proliferation. By analyzing recombinant clones, we also found that the viral hemagglutinin is responsible for the generation of suppressor T cells by reovirus type 3. These effects were observed whether UV-inactivated or live virus was used. Reovirus type 3 inhibition of the proliferative response of murine splenic lymphocytes to Con A was blocked by anti-reovirus type 3 antibody but not by anti-reovirus type 1 antibody. Antiviral antibody had no effect on the ability of reovirus type 3 induced suppressor cells to inhibit Con A proliferation. We have previously demonstrated a receptor on murine lymphocytes for the hemagglutinin of reovirus type 3, and our results suggest that the in vitro suppression of Con A proliferation of murine lymphocytes by reovirus type 3 is secondary to the interaction of the viral hemagglutinin with a receptor on the surface of murine lymphocytes, which results in the generation of functionally active suppressor T cells.
在研究呼肠孤病毒与淋巴细胞的相互作用时，我们发现3型呼肠孤病毒而非1型呼肠孤病毒可抑制小鼠脾淋巴细胞对刀豆蛋白A（Con A）的体外增殖反应。通过分析包含1型和3型呼肠孤病毒基因的重组克隆，我们发现S1基因（即编码病毒血凝素的基因）是造成这种抑制作用的原因。此外，我们还发现3型呼肠孤病毒而非1型呼肠孤病毒可在体外产生能够抑制Con A增殖的抑制性T细胞。通过分析重组克隆，我们还发现病毒血凝素是3型呼肠孤病毒产生抑制性T细胞的原因。无论使用紫外线灭活病毒还是活病毒，均可观察到这些效应。3型呼肠孤病毒对小鼠脾淋巴细胞Con A增殖反应的抑制作用可被抗3型呼肠孤病毒抗体阻断，但不能被抗1型呼肠孤病毒抗体阻断。抗病毒抗体对3型呼肠孤病毒诱导的抑制细胞抑制Con A增殖的能力没有影响。我们之前已证明小鼠淋巴细胞上存在3型呼肠孤病毒血凝素的受体，我们的结果表明，3型呼肠孤病毒对小鼠淋巴细胞Con A增殖的体外抑制作用继发于病毒血凝素与小鼠淋巴细胞表面受体的相互作用，这导致了功能活跃的抑制性T细胞的产生。
