胰蛋白酶对葡萄球菌肠毒素B与人胚胎小肠细胞培养物相互作用的干扰。
Interference by trypsin in the interaction of staphylococcal enterotoxin B and cell cultures of human embryonic intestine.
作者信息
Schaeffer W I, Gabliks J, Calitis R
出版信息
J Bacteriol. 1967 May;93(5):1489-92. doi: 10.1128/jb.93.5.1489-1492.1967.
Abstract
The inhibitory effect of trypsin on the cytotoxicity of staphylococcal enterotoxin B acting with human embryonic intestine cell cultures was examined. Trypsin treatment of the cells rendered them resistant to enterotoxin for a period of 48 hr. The resistance increased proportionally with increased time of exposure of the cells to trypsin. Neither ethylenediaminetetraacetic acid nor scraping, which were used as alternate means of cell suspension, caused any resistance to the toxin. The effect is enzymatic and appears to be similar to the inhibitory action of trypsin and chymotrypsin on the attachment of polioviruses and coxsackieviruses to HeLa cells.
摘要
研究了胰蛋白酶对葡萄球菌肠毒素B作用于人类胚胎肠细胞培养物的细胞毒性的抑制作用。用胰蛋白酶处理细胞可使它们在48小时内对肠毒素产生抗性。抗性随细胞暴露于胰蛋白酶的时间增加而成比例增加。用作细胞悬浮替代方法的乙二胺四乙酸和刮擦均未对毒素产生任何抗性。这种作用是酶促的，似乎类似于胰蛋白酶和糜蛋白酶对脊髓灰质炎病毒和柯萨奇病毒附着于HeLa细胞的抑制作用。
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