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磷脂酶C的改进检测方法。

Improved assay method for phospholipase C.

作者信息

Kurioka S, Liu P V

出版信息

Appl Microbiol. 1967 May;15(3):551-5. doi: 10.1128/am.15.3.551-555.1967.

DOI:10.1128/am.15.3.551-555.1967
PMID:4962285
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC546967/
Abstract

A lecithin sol dispersed with deoxycholate was found to be attacked by phospholipase C in the presence of calcium ion more rapidly than were any other lecithin sols. The inorganic phosphate could be released quantitatively from the acid soluble phosphate liberated from lecithin by an excess amount of alkaline phosphatase present in phospholipase C reaction mixture. A simple and accurate assay method for phospholipase C was developed with the sol and the alkaline phosphatase.

摘要

研究发现，在钙离子存在的情况下，分散有脱氧胆酸盐的卵磷脂溶胶比其他任何卵磷脂溶胶更易受到磷脂酶C的攻击。磷脂酶C反应混合物中过量存在的碱性磷酸酶可从卵磷脂释放的酸溶性磷酸盐中定量释放出无机磷酸盐。利用该溶胶和碱性磷酸酶开发了一种简单而准确的磷脂酶C检测方法。

相似文献

1
Improved assay method for phospholipase C.磷脂酶C的改进检测方法。
Appl Microbiol. 1967 May;15(3):551-5. doi: 10.1128/am.15.3.551-555.1967.
2
Synthesis of p-nitrophenylphosphorylcholine and the hydrolysis with phospholipase C.对硝基苯基磷酰胆碱的合成及磷脂酶C催化的水解反应
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3
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Hydrolytic action of phospholipases on bacterial membranes.磷脂酶对细菌细胞膜的水解作用。
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Apparent phosphate retrieval system in Bacillus cereus.蜡样芽孢杆菌中的表观磷酸盐回收系统。
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Lipase activity of detergent-resistant phospholipase A in Escherichia coli.大肠杆菌中耐去污剂磷脂酶A的脂肪酶活性
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8
Enzymatic hydrolysis by bacterial phospolipases C and D of immobilized radioactive sphingomyelin and phosphatidylcholine.细菌磷脂酶C和D对固定化放射性鞘磷脂和磷脂酰胆碱的酶促水解作用。
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Choline and betaine as inducer agents of Pseudomonas aeruginosa phospholipase C activity in high phosphate medium.胆碱和甜菜碱作为高磷酸盐培养基中铜绿假单胞菌磷脂酶C活性的诱导剂
FEMS Microbiol Lett. 1989 Feb;57(3):335-8. doi: 10.1016/0378-1097(89)90324-8.

引用本文的文献

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Infect Immun. 1999 Aug;67(8):4008-13. doi: 10.1128/IAI.67.8.4008-4013.1999.
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J Bacteriol. 1982 Oct;152(1):239-45. doi: 10.1128/jb.152.1.239-245.1982.
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Physicochemical fractionation of extracellular cornea-damaging proteases of Pseudomonas aeruginosa.铜绿假单胞菌细胞外角膜损伤蛋白酶的物理化学分级分离
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本文引用的文献

1
The biochemistry of bacterial toxins: The lecithinase activity of Cl. welchii toxins.细菌毒素的生物化学：产气荚膜梭菌毒素的卵磷脂酶活性。
Biochem J. 1941 Sep;35(8-9):884-902. doi: 10.1042/bj0350884.
2
Phospholipase C determination by egg yolk turbidimetry.采用蛋黄比浊法测定磷脂酶C
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3
Electrokinetic requirements for the reaction between Cl. perfringens alpha-toxin (phospholipase C) and phospholipid substrates.产气荚膜梭菌α毒素（磷脂酶C）与磷脂底物反应的电动学要求。
Biochim Biophys Acta. 1962 May 7;59:103-15. doi: 10.1016/0006-3002(62)90701-1.
4
Effects of solvents and surface-active agents on plastid phosphatidase C activity.溶剂和表面活性剂对质体磷脂酶C活性的影响。
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Enzymic hydrolysis of sphingomyelin by rat liver.大鼠肝脏对鞘磷脂的酶促水解作用。
Biochem J. 1966 Mar;98(3):763-9. doi: 10.1042/bj0980763.
6
The roles of various fractions of Pseudomonas aeruginosa in its pathogenesis. II. Effects of lecithinase and protease.铜绿假单胞菌各组分在其致病机制中的作用。II. 卵磷脂酶和蛋白酶的作用
J Infect Dis. 1966 Feb;116(1):112-6. doi: 10.1093/infdis/116.1.112.