磷脂酶C的改进检测方法。
Improved assay method for phospholipase C.
作者信息
Kurioka S, Liu P V
出版信息
Appl Microbiol. 1967 May;15(3):551-5. doi: 10.1128/am.15.3.551-555.1967.
Abstract
A lecithin sol dispersed with deoxycholate was found to be attacked by phospholipase C in the presence of calcium ion more rapidly than were any other lecithin sols. The inorganic phosphate could be released quantitatively from the acid soluble phosphate liberated from lecithin by an excess amount of alkaline phosphatase present in phospholipase C reaction mixture. A simple and accurate assay method for phospholipase C was developed with the sol and the alkaline phosphatase.
摘要
研究发现，在钙离子存在的情况下，分散有脱氧胆酸盐的卵磷脂溶胶比其他任何卵磷脂溶胶更易受到磷脂酶C的攻击。磷脂酶C反应混合物中过量存在的碱性磷酸酶可从卵磷脂释放的酸溶性磷酸盐中定量释放出无机磷酸盐。利用该溶胶和碱性磷酸酶开发了一种简单而准确的磷脂酶C检测方法。
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