Péry P, Luffau G, Charley J, Petit A, Rouze P, Bernard S
Ann Immunol (Paris). 1979 Jul-Aug;130C(4):517-29.
The oxidation of the ribose ring of cytidine-5'-diphospho-choline (CDPC) by periodate to produce reactive dialdehydes was used to couple this activated reagent onto various proteins, onto Ficoll 400 and onto Sepharose 4B. Careful control of parameters of the different steps of the reaction enabled us to synthesize conjugates with a graded number of nucleotide residues. Human serum albumin conjugates with a relatively high degree of substitution were used to demonstrate and to measure anti-phosphorylcholine antibodies and the acute phase reactant, C-reactive protein by precipitation or passive haemagglutination techniques. These methods for measuring C-reactive protein in serum of patient suffering from acute inflammation may be useful for clinicians. CDPC-AH Sepharose was used to purify the phosphorylcholine-binding myeloma protein HOPC8 and to separate C-reactive protein from the bulk of serum proteins. Improvements of this technique will certainly lead to the complete purification of C-reactive protein.
用高碘酸盐氧化胞苷-5'-二磷酸胆碱（CDPC）的核糖环以产生反应性二醛，从而将这种活化试剂偶联到各种蛋白质、聚蔗糖400和琼脂糖4B上。对反应不同步骤的参数进行仔细控制，使我们能够合成具有不同数量核苷酸残基的缀合物。具有相对较高取代度的人血清白蛋白缀合物用于通过沉淀或被动血凝技术来检测和测量抗磷酸胆碱抗体以及急性期反应物C反应蛋白。这些用于测量急性炎症患者血清中C反应蛋白的方法可能对临床医生有用。CDPC-AH琼脂糖用于纯化结合磷酸胆碱的骨髓瘤蛋白HOPC8，并从大量血清蛋白中分离出C反应蛋白。这项技术的改进肯定会导致C反应蛋白的完全纯化。
