Brown A R, Crandall C A
J Immunol. 1976 Apr;116(4):1105-9.
A serum component, binding antigens having phosphorylcholine (PC) determinants were induced in several strains of mice by infection with Ascaris suum. This component was isolated and demonstrated to be an IgM (K) anti-PC antibody having idiotypic determinants in common with the IgA PC-binding myeloma protein TEPC 15. Rabbit anti-idiotypic antisera prepared with this component had idiotypic specificity for TEPC 15 and cross-idiotypic recognition of MOPC 167 and McPC 603, all IgA PC-binding myeloma proteins. The antisera also recognized determinants not present on TEPC 15. IgM and idiotype levels were quantitated by radial immunodiffusion and PC-specific antibody measured by hemagglutination (HA) with sheep erythrocytes coated with pneumococcal-C-polysaccharide. Mean IgM levels ranged from 2.5 to 8.7 mg/ml, idiotype from 0.54 to 5.3 mg/ml; and HA titers from 1:512 to 1:130,000 in different mouse strains. The high PC-specific antibody response was not duplicated by immunization with dead ascaris larvae or by infection with two other nematode species.
通过猪蛔虫感染，在数种小鼠品系中诱导出一种血清成分，该成分可结合具有磷酸胆碱（PC）决定簇的抗原。分离出该成分，并证明其为一种IgM（κ）抗PC抗体，具有与IgA PC结合骨髓瘤蛋白TEPC 15相同的独特型决定簇。用该成分制备的兔抗独特型抗血清对TEPC 15具有独特型特异性，对所有IgA PC结合骨髓瘤蛋白MOPC 167和McPC 603具有交叉独特型识别能力。这些抗血清还识别TEPC 15上不存在的决定簇。通过放射免疫扩散法定量IgM和独特型水平，用包被有肺炎球菌C多糖的绵羊红细胞进行血凝试验（HA）测定PC特异性抗体。在不同小鼠品系中，平均IgM水平为2.5至8.7mg/ml，独特型为0.54至5.3mg/ml；HA效价为1:512至1:130,000。用死蛔虫幼虫免疫或感染另外两种线虫物种均未重复出现高PC特异性抗体反应。
