Henson P M
Immunology. 1967 Sep;13(3):261-74.
Three manifestations of the antibody response of rabbits and guinea-pigs to injections of killed bacteria have been examined, namely, complement-fixing and agglutinating anti-bacterial antibodies and immunoconglutinins (I-Ks) directed against fixed complement. Animals given intramuscular injections of the bacteria in Freund's complete adjuvant before a course of intravenous injections were compared with those receiving the latter course only. The prior immunization with organisms in adjuvant increased the I-K responses in rabbits. This was correlated with an increase in that part of the complement-fixing, anti-bacterial antibody contained in the IgG fraction. The animals receiving the course of intravenous injections only, produced more agglutinating anti-bacterial antibody (which was shown to be predominantly IgM in type) and less I-K. In guinea-pigs there was little difference in the I-K response between animals receiving the two injection schedules. The prior immunization with organisms in adjuvant did produce higher levels of anti-bacterial antibody, but in this species the increase was mainly in the IgM fraction. It is suggested that in both species the presence of complement-fixing anti-bacterial antibody of IgG immunoglobulin type is necessary for the stimulation of I-K production and that high levels of IgM antibody may have an inhibitory effect. High titres of I-K can be produced regularly by injecting bacteria intravenously into rabbits with predominantly IgG anti-bacterial antibodies. Where these do not occur naturally they can be produced in response to two intramuscular injections of bacteria in adjuvant. In guinea-pigs it was difficult to produce predominantly IgG anti-bacterial antibody and high titres of I-K were difficult to achieve.
已对家兔和豚鼠针对注射死菌的抗体反应的三种表现形式进行了研究，即补体结合和凝集抗菌抗体以及针对固定补体的免疫粘连素（I-Ks）。将在静脉注射疗程前接受弗氏完全佐剂中细菌肌肉注射的动物与仅接受后一疗程的动物进行了比较。佐剂中生物体的预先免疫增加了家兔的I-K反应。这与IgG组分中所含补体结合抗菌抗体的那部分增加相关。仅接受静脉注射疗程的动物产生了更多的凝集抗菌抗体（已证明其主要为IgM型），而I-K较少。在豚鼠中，接受两种注射方案的动物之间的I-K反应几乎没有差异。佐剂中生物体的预先免疫确实产生了更高水平的抗菌抗体，但在该物种中，增加主要在IgM组分中。有人提出，在这两个物种中，IgG免疫球蛋白类型的补体结合抗菌抗体的存在对于刺激I-K产生是必要的，而高水平的IgM抗体可能具有抑制作用。通过向具有主要为IgG抗菌抗体的家兔静脉注射细菌，可以定期产生高滴度的I-K。在自然情况下不存在这些抗体的地方，可以通过对佐剂中的细菌进行两次肌肉注射来产生它们。在豚鼠中，很难产生主要为IgG的抗菌抗体，并且很难达到高滴度的I-K。
