铜绿假单胞菌的细胞壁。一般组成。
The cell walls of Pseudomonas aeruginosa. General composition.
作者信息
Clarke K, Gray G W, Reaveley D A
出版信息
Biochem J. 1967 Nov;105(2):749-54. doi: 10.1042/bj1050749.
Abstract
- Cell walls of Pseudomonas aeruginosa were prepared and analysed. 2. Separate preparations were found to be reproducible, e.g. the phosphorus contents of all batches lay in the range 2.0-2.1%. 3. Ninhydrin-positive compounds in hydrolysates accounted for 43-46% of the cell wall and 79-87% of the nitrogen of the cell wall. Examination of the results for individual ninhydrin-positive compounds showed that 5-15% of the cell wall was murein and about 30% protein. 4. Trypsin treatment of crude cell-wall preparations preferentially liberated arginine, lysine and leucine, but it was not clear whether these arose from cell-wall or cytoplasmic proteins.
摘要
- 制备并分析了铜绿假单胞菌的细胞壁。2. 发现不同的制备物具有可重复性，例如所有批次的磷含量在2.0 - 2.1%范围内。3. 水解产物中茚三酮阳性化合物占细胞壁的43 - 46%，占细胞壁氮的79 - 87%。对单个茚三酮阳性化合物的结果检查表明，细胞壁的5 - 15%是胞壁质，约30%是蛋白质。4. 用胰蛋白酶处理粗制细胞壁制剂时，优先释放出精氨酸、赖氨酸和亮氨酸，但尚不清楚这些是来自细胞壁蛋白还是细胞质蛋白。
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