用苯酚水溶液提取铜绿假单胞菌的细胞壁。来自苯酚层的物质。
The extraction of cell walls of Pseudomonas aeruginosa with aqueous phenol. Material from the phenol layer.
作者信息
Clarke K, Gray G W, Reaveley D A
出版信息
Biochem J. 1967 Nov;105(2):755-8. doi: 10.1042/bj1050755.
Abstract
- A method is described for the fractionation of cell walls of Pseudomonas aeruginosa by using aqueous phenol. 2. With this technique, cell walls are quantitatively separated into five fractions: two water-soluble fractions (AqI and AqII), a residual insoluble fraction (R), and two phenol-soluble fractions (PhMP and PhMS). 3. The compositions of fractions PhMP and PhMS are discussed. Fraction PhMP consists almost entirely of protein, which is soluble in aqueous sodium dodecyl sulphate. Analytical ultracentrifugation of the solution yields a single peak, but several components may be resolved by gel electrophoresis. 4. Fraction PhMS consists principally of phospholipid (approx. 44%) and fatty acids (approx. 27%), although smaller amounts (approx. 13%) of protein are present.
摘要
- 描述了一种使用苯酚水溶液对铜绿假单胞菌细胞壁进行分级分离的方法。2. 采用该技术，细胞壁可定量分离为五个级分：两个水溶性级分（AqI和AqII）、一个残留不溶级分（R）以及两个酚溶性级分（PhMP和PhMS）。3. 讨论了PhMP和PhMS级分的组成。PhMP级分几乎完全由可溶于十二烷基硫酸钠水溶液的蛋白质组成。对该溶液进行分析超速离心产生一个单峰，但通过凝胶电泳可分辨出几种成分。4. PhMS级分主要由磷脂（约44%）和脂肪酸（约27%）组成，不过也含有少量（约13%）蛋白质。
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